Nuclear Red™ DCS1 5 mM DMSO Solution

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Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
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Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	671.42
Solvent	DMSO

Spectral properties

Excitation (nm)	631
Emission (nm)	651

Storage, safety and handling

Certificate of Origin	Download PDF
H-phrase	H303, H313, H340
Hazard symbol	T
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R68
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	41116134

Alternative formats

Nuclear Orange™ DCS1 *5 mM DMSO Solution*

Overview SDS Protocol

Molecular weight

671.42

Excitation (nm)

631

Emission (nm)

651

Our Nuclear Red™ DCS1 is a fluorogenic, DNA-selective and cell-impermeant dye for analyzing DNA content in dead, fixed or apoptotic cells. The Nuclear Red™ DCS1 has its red fluorescence significantly enhanced upon binding to DNA. It can be used in fluorescence imaging, microplate and flow cytometry applications. This DNA-binding dye might be used for multicolor analysis of dead, fixed or apoptotic cells with proper filter sets.

Platform

Fluorescence microscope

Excitation	Cy5 Filter
Emission	Cy5 Filter
Recommended plate	Black wall/clear bottom

Example protocol

AT A GLANCE

Spectral Properties

Ex/Em = 631/651 nm (bound to DNA)

SAMPLE EXPERIMENTAL PROTOCOL

Caution: The following protocol can be adapted for most cell types. Growth medium, cell density, the presence of other cell types, and factors may influence staining. Residual detergent on glassware may also affect the staining of many organisms and cause brightly stained material to appear in solutions with or without cells present.

Add Nuclear Red™ DCS1 (2 to10 µM) into the fixed, dead or apoptotic cells (either suspension or adherent) and incubate the cells for 15 to 60 minutes.

Note: In initial experiments, it is advisable to test a wide range of dye concentrations in order to determine the optimal concentration that yields the desired result.

Optional: Wash the cells twice with Hanks and 20 mM HEPES buffer (HBSS) or a buffer of your choice. Then fill the wells with fresh HBSS or growth medium.
Observe the cells using a fluorescence microscope, fluorescence microplate reader, or flow cytometer equipped with the desired filter set.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Nuclear Red™ DCS1 *5 mM DMSO Solution* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	148.938 µL	744.69 µL	1.489 mL	7.447 mL	14.894 mL
5 mM	29.788 µL	148.938 µL	297.876 µL	1.489 mL	2.979 mL
10 mM	14.894 µL	74.469 µL	148.938 µL	744.69 µL	1.489 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Spectrum

Open in Advanced Spectrum Viewer

Spectral properties

Excitation (nm)	631
Emission (nm)	651

Product Family

Name	Excitation (nm)	Emission (nm)
Nuclear Blue™ DCS1 5 mM DMSO Solution	348	469
Nuclear Green™ DCS1 5 mM DMSO Solution	503	527
Nuclear Orange™ DCS1 5 mM DMSO Solution	514	556

Images

Figure 1. Fluorescence image of HeLa cells fixed with 4% formaldehyde then stained with iFluor 488 Phalloidin (Cat#23115, Green) and nuclei stain Nuclear Red™ DCS1 (Cat#17552, Red)

Citations

View all 9 citations: Citation Explorer

Nuclear translocation of Axl contributes to the malignancy of oral cancer cells
Authors: Su, Yu-Fu and Shen, Po-Chien and Huang, Wen-Yen and Hung, Yi-Jen and Huang, Tsai-Wang and Lin, Che-Yi and Shieh, Yi-Shing
Journal: Journal of Dental Sciences (2023)

Bezafibrate Exerts Neuroprotective Effects in a Rat Model of Sporadic Alzheimer’s Disease
Authors: Lin, Li-Fan and Jhao, Yun-Ting and Chiu, Chuang-Hsin and Sun, Lu-Han and Chou, Ta-Kai and Shiue, Chyng-Yann and Cheng, Cheng-Yi and Ma, Kuo-Hsing
Journal: Pharmaceuticals (2022): 109

Calcitonin induces bone formation by increasing expression of Wnt10b in osteoclasts in ovariectomy-induced osteoporotic rats
Authors: Hsiao, Chen-Yuan and Chen, Tien-Hua and Chu, Tzu-Hui and Ting, Yen-Nien and Tsai, Pei-Jiun and Shyu, Jia-Fwu
Journal: Frontiers in Endocrinology (2020): 613

Osteoclast-released Wnt-10b underlies cinacalcet related bone improvement in chronic kidney disease
Authors: Zheng, Cai-Mei and Hsu, Yung-Ho and Wu, Chia-Chao and Lu, Chien-Lin and Liu, Wen-Chih and Zheng, Jing-Quan and Lin, Yuh-Feng and Chiu, Hui-Wen and Chang, Tian-Jong and Shyu, Jia-Fwu and others,
Journal: International journal of molecular sciences (2019): 2800

Neural stem cells for disease modeling and evaluation of therapeutics for infantile (CLN1/PPT1) and late infantile (CLN2/TPP1) neuronal ceroid lipofuscinoses
Authors: Sima, Ni and Li, Rong and Huang, Wei and Xu, Miao and Beers, Jeanette and Zou, Jizhong and Titus, Steven and Ottinger, Elizabeth A and Marugan, Juan J and Xie, Xing and others, undefined
Journal: Orphanet journal of rare diseases (2018): 54

Synthesis of the Anionic Hydroxypropyl-β-cyclodextrin: Poly (decamethylenephosphate) Polyrotaxane and Evaluation of its Cholesterol Efflux Potential in Niemann-Pick C1 Cells
Authors: Egele, Kerstin and Samaddar, Shayak and Schneider, Nina and Thompson, David H and Wenz, Gerhard
Journal: Journal of Materials Chemistry B (2018)

Methyl-$\beta$-cyclodextrin restores impaired autophagy flux in Niemann-Pick C1-deficient cells through activation of AMPK
Authors: Dai, Sheng and Dulcey, Andr{\'e}s E and Hu, Xin and Wassif, Christopher A and Porter, Forbes D and Austin, Christopher P and Ory, Daniel S and Marugan, Juan and Zheng, Wei
Journal: Autophagy (2017): 1435--1451

One-Step Seeding of Neural Stem Cells with Vitronectin-Supplemented Medium for High-Throughput Screening Assays
Authors: Dai, Sheng and Li, Rong and Long, Yan and Titus, Steve and Zhao, Jinghua and Huang, Ruili and Xia, Menghang and Zheng, Wei
Journal: Journal of Biomolecular Screening (2016): 1112--1124

Genome shuffling of the nonconventional yeast Pichia anomala for improved sugar alcohol production
Authors: Zhang, Guoqiang and Lin, Yuping and Qi, Xianni and Wang, Lixian and He, Peng and Wang, Qinhong and Ma, Yanhe
Journal: Microbial cell factories (2015): 1

References

View all 65 references: Citation Explorer

In vitro activity of pyrvinium pamoate against Entamoeba histolytica and Giardia intestinalis using radiolabelled thymidine incorporation and an SYBR Green I-based fluorescence assay
Authors: Downey AS, Graczyk TK, Sullivan DJ.
Journal: J Antimicrob Chemother (2009): 751

Detection of methylation in hepatocellular carcinoma using SYBR Green fluorescent quantitative PCR
Authors: Yang B, Lou C, Gao Y, Du Z, Song W.
Journal: Zhonghua Yi Xue Yi Chuan Xue Za Zhi (2008): 534

Estimation of copy number using SYBR Green: confounding by AT-rich DNA and by variation in amplicon length
Authors: Colborn JM, Byrd BD, Koita OA, Krogstad DJ.
Journal: Am J Trop Med Hyg (2008): 887

DRAQ5-based, no-lyse, no-wash bone marrow aspirate evaluation by flow cytometry
Authors: Allan RW, Ansari-Lari MA, Jordan S.
Journal: Am J Clin Pathol (2008): 706

Interaction of a DNA intercalator DRAQ5, and a minor groove binder SYTO17, with chromatin in live cells--influence on chromatin organization and histone-DNA interactions
Authors: Wojcik K, Dobrucki JW.
Journal: Cytometry A (2008): 555

Addition of gold nanoparticles to real-time PCR: effect on PCR profile and SYBR Green I fluorescence
Authors: Haber AL, Griffiths KR, Jamting AK, Emslie KR.
Journal: Anal Bioanal Chem (2008): 887

Tetraalkylammonium derivatives as real-time PCR enhancers and stabilizers of the qPCR mixtures containing SYBR Green I
Authors: Shaik GM, Draberova L, Draber P, Boubelik M.
Journal: Nucleic Acids Res (2008): e93

Highly sensitive and selective detection of Hg(2+) in aqueous solution with mercury-specific DNA and Sybr Green I
Authors: Wang J, Liu B.
Journal: Chem Commun (Camb) (2008): 4759

Group testing to annihilate pairs applied to DNA cross-hybridization elimination using SYBR Green I
Authors: Bishop M, Macula AJ, Nimmo K, Wood L, Pogozelski WK, Renz TE.
Journal: J Comput Biol (2007): 84

DRAQ5: improved flow cytometric DNA content analysis and minimal residual disease detection in childhood malignancies
Authors: Swerts K, Van Roy N, Benoit Y, Laureys G, Philippe J.
Journal: Clin Chim Acta (2007): 154