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AAT Bioquest

PacBlue Anti-human CD1 Antibody *L161*

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Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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Spectral properties
Correction Factor (260 nm)0.15
Correction Factor (280 nm)0.2
Extinction coefficient (cm -1 M -1)46000
Excitation (nm)404
Emission (nm)455
Quantum yield0.78
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

OverviewpdfSDSpdfProtocol


Other names
R7; M241
Clone
L161
Host
mouse
Isotype
Mouse IgG1 kappa
Reactivity
human
Application
Flow Cytometry (FACS); Fluorescence Imaging
Correction Factor (260 nm)
0.15
Correction Factor (280 nm)
0.2
Extinction coefficient (cm -1 M -1)
46000
Excitation (nm)
404
Emission (nm)
455
Quantum yield
0.78
L161 is an anti-human monoclonal antibody that is specific for the CD1c antigen. CD1c (also known as M241 or R7) is a 43 kD glycoprotein that is expressed on the surface of cells such as dendritic cells, macrophages, B cells and T cells. In certain organisms, CD1 acts to positively regulate T cell mediated cytotoxicity, and is associated with a variety of biologically interesting macromolecules/ligands, for example, β-2-microglobulin. CD1 is a moderately popular antibody target, with over 15000 publications in the last decade. CD1c is essential for immunology research, typically serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to PacBlue (ex/em = 404/455 nm). It is compatible with the 405 nm laser and 450/50 nm bandpass filter (for example, as in the BD FACSCelesta™).

Spectrum


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spectrum

Spectral properties

Correction Factor (260 nm)0.15
Correction Factor (280 nm)0.2
Extinction coefficient (cm -1 M -1)46000
Excitation (nm)404
Emission (nm)455
Quantum yield0.78

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Conjugation


We provide custom conjugation services for this antibody (eg. labeling of cd1c antibody with HRP). A list of available labels can be found in the table below:

AFAF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
ProteinsHRP, Alkaline Phosphatase, Streptavidin
TandemsAPC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small MoleculesBiotin
Traditional DyesFITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluorUV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page here.