PacBlue Anti-human CD158d Antibody *mAb#33*
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Spectral properties
Correction Factor (260 nm) | 0.15 |
Correction Factor (280 nm) | 0.2 |
Extinction coefficient (cm -1 M -1) | 46000 |
Excitation (nm) | 404 |
Emission (nm) | 455 |
Quantum yield | 0.78 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Overview | SDSProtocol |
Other names KIR2DL4 | Clone mAb#33 | Host mouse | Isotype Mouse IgG1 kappa | Reactivity human | Application Flow Cytometry (FACS); Fluorescence Imaging | Correction Factor (260 nm) 0.15 | Correction Factor (280 nm) 0.2 | Extinction coefficient (cm -1 M -1) 46000 | Excitation (nm) 404 | Emission (nm) 455 | Quantum yield 0.78 |
The mAb#33 monoclonal antibody binds to human CD158d, a 45 - 50 kD transmembrane glycoprotein frequently expressed on the surface of natural killer cells. In certain organisms, CD158d negatively regulates natural killer cell mediated cytotoxicity, is an enhancer of cellular senescence and positively regulates natural killer cell cytokine production. From a research standpoint, it is of biological interest due to its association with essential macromolecules/ligands like HLA-Bw4. CD158d is a relatively rare antibody target, with fewer than 200 publications in the last decade. Even still, CD158d has been widely used in immunology and innate immunity research, often serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to PacBlue (ex/em = 404/455 nm). It is compatible with the 405 nm laser and 440/50 nm bandpass filter (for example, as in the Thermo Fisher Attune NxT).
Spectrum
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Spectral properties
Correction Factor (260 nm) | 0.15 |
Correction Factor (280 nm) | 0.2 |
Extinction coefficient (cm -1 M -1) | 46000 |
Excitation (nm) | 404 |
Emission (nm) | 455 |
Quantum yield | 0.78 |
Antibody Finder
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Conjugation
We provide custom conjugation services for this antibody (eg. labeling of cd158d antibody with HRP). A list of available labels can be found in the table below:
For additional information about custom conjugations, please consult our services page here.
AF | AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750 |
Proteins | HRP, Alkaline Phosphatase, Streptavidin |
Tandems | APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5 |
Small Molecules | Biotin |
Traditional Dyes | FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5 |
iFluor | 350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7 |
mFluor | UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780 |
For additional information about custom conjugations, please consult our services page here.
Application notes
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Biotin Labeling Molecules and Their Biological Applications
Buccutite™ Bioconjugation Technology
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Biotin Labeling Molecules and Their Biological Applications
Buccutite™ Bioconjugation Technology
FAQ
How can I lyse my cells without lysing the nuclear membrane?
What are the differences between calcium ion indicators: Cal 520, Cal 520FF, and Cal 520N?
How do I make an AM ester stock solution?
Can we fix cells with glutaraldehyde and then stain with fluorescent phalloidin?
What is the difference between FluoroQuest Anti-fading Kit I and FluoroQuest Anti-fading Kit II?
What are the differences between calcium ion indicators: Cal 520, Cal 520FF, and Cal 520N?
How do I make an AM ester stock solution?
Can we fix cells with glutaraldehyde and then stain with fluorescent phalloidin?
What is the difference between FluoroQuest Anti-fading Kit I and FluoroQuest Anti-fading Kit II?