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PacBlue Anti-human CD19 Antibody
SJ25C1
SJ25C1 is an anti-human monoclonal antibody that forms an immune complex with the CD19 antigen. CD19 (sometimes called T-cell surface antigen Leu-12 or B-lymphocyte surface antigen B4) is a 95 kD transmembrane glycoprotein that is located on the surface of cells such as stem cells, B cells and dendritic cells. CD19 is a member of key cellular pathways, in particular, the antigen receptor-mediated signaling pathway and B cell receptor signaling pathway. Additionally, in many organisms, it upregulates release of sequestered calcium ion into cytosol, promotes protein kinase B signaling and is a promoter of phosphatidylinositol 3-kinase activity. From a research standpoint, it is of biological interest due to its association with key macromolecules/ligands such as CD225. CD19 is a very popular antibody target, with over 36000 publications in the last decade. CD19 is often used in flow cytometry applications as a phenotypic marker for differentiation of cell types, particularly in the study of immunology and costimulatory molecules. This antibody was purified through affinity chromatography and conjugated to PacBlue (ex/em = 404/455 nm). It is compatible with the 405 nm laser and 450/50 nm bandpass filter (for example, as in the BD FACSAria™ Fusion).
| Catalog | Size | Price | Quantity |
|---|---|---|---|
| 101911J0 | 100 tests | Price | |
| 101911J1 | 500 tests | Price |
Antibody properties
| Other names | B4 |
| Clone | SJ25C1 |
| Host | mouse |
| Reactivity | human |
| Application | Flow Cytometry (FACS); Fluorescence Imaging |
Spectral properties
| Correction factor (260 nm) | 0.15 |
| Correction factor (280 nm) | 0.2 |
| Extinction coefficient (cm -1 M -1) | 46000 |
| Excitation (nm) | 404 |
| Emission (nm) | 455 |
| Quantum yield | 0.78 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
Contact us
| Telephone | |
| Fax | |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Technical Support | Contact us |
| Request quotation | Request |
| Purchase order | Send to sales@aatbio.com |
| Shipping | Standard overnight for United States, inquire for international |
Page updated on October 15, 2025