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PacBlue Anti-human CD19 Antibody *SJ25C1*

SJ25C1 is an anti-human monoclonal antibody that forms an immune complex with the CD19 antigen. CD19 (sometimes called T-cell surface antigen Leu-12 or B-lymphocyte surface antigen B4) is a 95 kD transmembrane glycoprotein that is located on the surface of cells such as stem cells, B cells and dendritic cells. CD19 is a member of key cellular pathways, in particular, the antigen receptor-mediated signaling pathway and B cell receptor signaling pathway. Additionally, in many organisms, it upregulates release of sequestered calcium ion into cytosol, promotes protein kinase B signaling and is a promoter of phosphatidylinositol 3-kinase activity. From a research standpoint, it is of biological interest due to its association with key macromolecules/ligands such as CD225. CD19 is a very popular antibody target, with over 36000 publications in the last decade. CD19 is often used in flow cytometry applications as a phenotypic marker for differentiation of cell types, particularly in the study of immunology and costimulatory molecules. This antibody was purified through affinity chromatography and conjugated to PacBlue (ex/em = 404/455 nm). It is compatible with the 405 nm laser and 450/50 nm bandpass filter (for example, as in the BD FACSAria™ Fusion).

Spectrum

Conjugation

We provide custom conjugation services for this antibody (eg. labeling of cd19 antibody with HRP). A list of available labels can be found in the table below:

AFAF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
ProteinsHRP, Alkaline Phosphatase, Streptavidin
TandemsAPC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small MoleculesBiotin
Traditional DyesFITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluorUV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page here.
Page updated on June 26, 2025

Ordering information

Price
Unit size
100 tests
500 tests
Catalog Number
101911J0101911J1
Quantity
Add to cart

Additional ordering information

Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
InternationalSee distributors
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Custom sizeInquire
Technical SupportContact us
Purchase orderSend to sales@aatbio.com
ShippingStandard overnight for United States, inquire for international
Request quotation

Antibody properties

Other namesB4
CloneSJ25C1
Hostmouse
Reactivityhuman
ApplicationFlow Cytometry (FACS); Fluorescence Imaging

Spectral properties

Correction Factor (260 nm)

0.15

Correction Factor (280 nm)

0.2

Extinction coefficient (cm -1 M -1)

46000

Excitation (nm)

404

Emission (nm)

455

Quantum yield

0.78

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
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