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PacBlue Anti-human CD253 Antibody
2E5
2E5 is an anti-human monoclonal antibody that recognizes the CD253 antigen. CD253 (sometimes called Apo-2L, TRAIL or TNFSF10) is a 30 kD member of the TNF ligand superfamily that is expressed on the surface of cells such as B cells and macrophages. CD253 is associated with a variety of biologically interesting macromolecules/ligands, for example, apo2. CD253 is a relatively rare antibody target, with fewer than 100 publications in the last decade. Even still, CD253 is essential for immunology research, frequently serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to PacBlue (ex/em = 404/455 nm). It is compatible with the 405 nm laser and 440/50 nm bandpass filter (for example, as in the Thermo Fisher Attune NxT).
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CatalogSize
Price
Quantity
125301K0100 tests
Price
125301K1500 tests
Price
 
Antibody properties

Other namesTRAIL, Apo-2L, TNFSF10
Clone2E5
HostMouse
IsotypeIgG1
ReactivityHuman
ApplicationFlow Cytometry (FACS), Fluorescence Imaging
Spectral properties

Correction factor (260 nm)0.15
Correction factor (280 nm)0.2
Extinction coefficient (cm -1 M -1)
46000
Excitation (nm)404
Emission (nm)455
Quantum yield
0.78
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
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Page updated on June 5, 2026