PacBlue Anti-human CD314 Antibody 1D11

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Spectral properties

Correction Factor (260 nm)	0.15
Correction Factor (280 nm)	0.2
Extinction coefficient (cm ^-1 M ^-1)	46000
Excitation (nm)	404
Emission (nm)	455
Quantum yield	0.78

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22

Overview SDS Protocol

Other names

NKG2D

Clone

1D11

Host

mouse

Isotype

Mouse IgG1 kappa

Reactivity

human

Application

Flow Cytometry (FACS); Fluorescence Imaging

Correction Factor (260 nm)

0.15

Correction Factor (280 nm)

0.2

Extinction coefficient (cm ^-1 M ^-1)

46000

Excitation (nm)

404

Emission (nm)

455

Quantum yield

0.78

The 1D11 monoclonal antibody binds to human CD314, a transmembrane protein commonly found on the surface of natural killer cells and T cells. CD314 is associated with a variety of biologically interesting macromolecules/ligands, namely, ULBP2, MICB, ULBP1 and MICA. CD314 is a relatively rare antibody target, with fewer than 300 publications in the last decade. Even still, CD314 is essential for costimulatory molecules research, typically serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to PacBlue (ex/em = 404/455 nm). It is compatible with the 405 nm laser and 457/45 nm bandpass filter (for example, as in the Luminex Amnis ImageStream).

Spectrum

Open in Advanced Spectrum Viewer

Spectral properties

Correction Factor (260 nm)	0.15
Correction Factor (280 nm)	0.2
Extinction coefficient (cm ^-1 M ^-1)	46000
Excitation (nm)	404
Emission (nm)	455
Quantum yield	0.78

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Conjugation

We provide custom conjugation services for this antibody (eg. labeling of cd314 antibody with HRP). A list of available labels can be found in the table below:

AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780