PacBlue Anti-human CD318 Antibody CUB1

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Spectral properties

Correction Factor (260 nm)	0.15
Correction Factor (280 nm)	0.2
Extinction coefficient (cm ^-1 M ^-1)	46000
Excitation (nm)	404
Emission (nm)	455
Quantum yield	0.78

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22

Overview SDS Protocol

Other names

CDCP1; SIMA135

Clone

CUB1

Host

mouse

Isotype

Mouse IgG2b

Reactivity

human

Application

Flow Cytometry (FACS); Fluorescence Imaging

Correction Factor (260 nm)

0.15

Correction Factor (280 nm)

0.2

Extinction coefficient (cm ^-1 M ^-1)

46000

Excitation (nm)

404

Emission (nm)

455

Quantum yield

0.78

CUB1 is an anti-human monoclonal antibody that recognizes the CD318 antigen. CD318 (also known as CDCP1) is a 93 kD single-pass membrane protein that is found on the surface of cells like stem cells. CD318 is associated with a variety of biologically interesting macromolecules/ligands, in particular, N-Caherin and syndecan-4. CD318 is a relatively rare antibody target, with fewer than 60 publications in the last decade. Even still, CD318 is vital to neuroscience cell markers, neuroscience and stem cells research, often serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to PacBlue (ex/em = 404/455 nm). It is compatible with the 405 nm laser and 445/45 nm bandpass filter (for example, as in the Agilent Technologies NovoCyte).

Spectrum

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Spectral properties

Correction Factor (260 nm)	0.15
Correction Factor (280 nm)	0.2
Extinction coefficient (cm ^-1 M ^-1)	46000
Excitation (nm)	404
Emission (nm)	455
Quantum yield	0.78

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Conjugation

We provide custom conjugation services for this antibody (eg. labeling of cd318 antibody with HRP). A list of available labels can be found in the table below:

AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780