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PacBlue Anti-human CD45 Antibody *HI73*

HI73 is an anti-human monoclonal antibody that recognizes the CD45 antigen. CD45 (also known as PTPRC, Leukocyte common antigen or T200) is a 180 - 240 kD transmembrane glycoprotein that is found on the surface of cells like stem cells, dendritic cells and B cells. CD45 has been associated with important biological processes such as dephosphorylation, especially protein dephosphorylation. Additionally, in certain organisms, it positively regulates tumor necrosis factor production, promotes stem cell proliferation and promotes isotype switching to IgG isotypes. CD45 is involved with key cellular pathways, for example, the B cell receptor signaling pathway, T cell receptor signaling pathway and negative regulation of cytokine-mediated signaling pathway. From a research standpoint, it is of biological interest due to its association with critical macromolecules/ligands such as p56lck, Src kinases and p59fyn. CD45 is a very popular antibody target, with over 50000 publications in the last decade. CD45 has a variety of applications in cell biology and inhibitory molecules research, typically serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to PacBlue (ex/em = 404/455 nm). It is compatible with the 405 nm laser and 450/50 nm bandpass filter (for example, as in the BD Special Order LSRFortessa™ Cell Analyzer).

Spectrum

Conjugation

We provide custom conjugation services for this antibody (eg. labeling of cd45 antibody with HRP). A list of available labels can be found in the table below:

AFAF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
ProteinsHRP, Alkaline Phosphatase, Streptavidin
TandemsAPC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small MoleculesBiotin
Traditional DyesFITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluorUV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page here.
Page updated on September 16, 2024

Ordering information

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Additional ordering information

Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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Antibody properties

Other namesLeukocyte Common Antigen (LCA); T200; PTPRC
CloneHI73
Hostmouse
Isotypemouse IgG2a
Reactivityhuman
ApplicationFlow Cytometry (FACS); Fluorescence Imaging

Spectral properties

Correction Factor (260 nm)

0.15

Correction Factor (280 nm)

0.2

Extinction coefficient (cm -1 M -1)

46000

Excitation (nm)

404

Emission (nm)

455

Quantum yield

0.78

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
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