PacBlue Anti-human CD5 Antibody L17F12

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Spectral properties

Correction Factor (260 nm)	0.15
Correction Factor (280 nm)	0.2
Extinction coefficient (cm ^-1 M ^-1)	46000
Excitation (nm)	404
Emission (nm)	455
Quantum yield	0.78

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22

Overview SDS Protocol

Other names

Leu1; Ly-1; T1; Tp67

Clone

L17F12

Host

mouse

Reactivity

human

Application

Flow Cytometry (FACS); Fluorescence Imaging

Correction Factor (260 nm)

0.15

Correction Factor (280 nm)

0.2

Extinction coefficient (cm ^-1 M ^-1)

46000

Excitation (nm)

404

Emission (nm)

455

Quantum yield

0.78

L17F12 is an anti-human monoclonal antibody that is specific for the CD5 antigen. CD5 (also known as Lyt-1 or Ly-1) is a 67 kD single-pass type I membrane protein that is found on the surface of cells such as B cells and T cells. CD5 is a component of important cellular pathways, namely, the apoptotic signaling pathway. From a research standpoint, it is of biological interest due to its association with essential macromolecules/ligands such as gp35-37, TCR and CD21. CD5 is a moderately popular antibody target, with over 11000 publications in the last decade. CD5 is typically used in flow cytometry applications as a phenotypic marker for differentiation of cell types, especially in the study of immunology. This antibody was purified through affinity chromatography and conjugated to PacBlue (ex/em = 404/455 nm). It is compatible with the 405 nm laser and 450/50 nm bandpass filter (for example, as in the Miltenyi Biotec MACSQuant X).

Spectrum

Open in Advanced Spectrum Viewer

Spectral properties

Correction Factor (260 nm)	0.15
Correction Factor (280 nm)	0.2
Extinction coefficient (cm ^-1 M ^-1)	46000
Excitation (nm)	404
Emission (nm)	455
Quantum yield	0.78

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Conjugation

We provide custom conjugation services for this antibody (eg. labeling of cd5 antibody with HRP). A list of available labels can be found in the table below:

AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780