PacBlue Anti-human CD62 Antibody *HI62E*
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Spectral properties
Correction Factor (260 nm) | 0.15 |
Correction Factor (280 nm) | 0.2 |
Extinction coefficient (cm -1 M -1) | 46000 |
Excitation (nm) | 404 |
Emission (nm) | 455 |
Quantum yield | 0.78 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Overview | SDSProtocol |
Other names E-selectin; ELAM-1 | Clone HI62E | Host mouse | Isotype mouse IgG2a | Reactivity human | Application Flow Cytometry (FACS); Fluorescence Imaging | Correction Factor (260 nm) 0.15 | Correction Factor (280 nm) 0.2 | Extinction coefficient (cm -1 M -1) 46000 | Excitation (nm) 404 | Emission (nm) 455 | Quantum yield 0.78 |
The HI62E monoclonal antibody reacts with human CD62e, a 115 kD single-pass type i membrane protein frequently expressed on the surface of endothelial cells and tnf α)s. CD62 has been associated with vital biological processes like inflammatory response, particularly leukocyte migration involved in inflammatory response. Also, in many organisms, it is an enhancer of receptor internalization. From a research standpoint, it is of biological interest due to its association with key macromolecules/ligands such as a and Sialyl Lewis x. CD62 is a relatively rare antibody target, with fewer than FAKE103000FAKE publications in the last decade. Even still, CD62e is often used in flow cytometry applications as a phenotypic marker for differentiation of cell types, specifically in the study of cell biology, neuroscience cell markers and immunology. This antibody was purified through affinity chromatography and conjugated to PacBlue (ex/em = 404/455 nm). It is compatible with the 405 nm laser and 450/50 nm bandpass filter (for example, as in the BD FACSCanto™).
Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
Correction Factor (260 nm) | 0.15 |
Correction Factor (280 nm) | 0.2 |
Extinction coefficient (cm -1 M -1) | 46000 |
Excitation (nm) | 404 |
Emission (nm) | 455 |
Quantum yield | 0.78 |
Antibody Finder
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Conjugation
We provide custom conjugation services for this antibody (eg. labeling of cd62e antibody with HRP). A list of available labels can be found in the table below:
For additional information about custom conjugations, please consult our services page here.
AF | AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750 |
Proteins | HRP, Alkaline Phosphatase, Streptavidin |
Tandems | APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5 |
Small Molecules | Biotin |
Traditional Dyes | FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5 |
iFluor | 350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7 |
mFluor | UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780 |
For additional information about custom conjugations, please consult our services page here.
Application notes
A Meta-Analysis of Common Calcium Indicators
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Abbreviation of Common Chemical Compounds Related to Peptides
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Abbreviation of Common Chemical Compounds Related to Peptides
FAQ
Are Cell Navigator® Cell Plasma Membrane Staining Kits suitable for cell culture medium samples?
Are there any alternatives to BrdU (Bromodeoxyuridine)?
Are there any alternatives to Cy5?
Are there any alternatives to indocyanine green (ICG)?
Are there any calcium indicators that don't require probenecid (PBC)?
Are there any alternatives to BrdU (Bromodeoxyuridine)?
Are there any alternatives to Cy5?
Are there any alternatives to indocyanine green (ICG)?
Are there any calcium indicators that don't require probenecid (PBC)?