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AAT Bioquest

PacOrange Anti-human CD180 Antibody *G28-8*

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Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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Spectral properties
Correction Factor (280 nm)0.6
Extinction coefficient (cm -1 M -1)25000
Excitation (nm)400
Emission (nm)551
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

OverviewpdfSDSpdfProtocol


Other names
RP105
Clone
G28-8
Host
mouse
Isotype
Mouse IgG1 kappa
Reactivity
human
Application
Flow Cytometry (FACS); Fluorescence Imaging
Correction Factor (280 nm)
0.6
Extinction coefficient (cm -1 M -1)
25000
Excitation (nm)
400
Emission (nm)
551
The G28-8 monoclonal antibody binds with human CD180, a 105 kD transmembrane protein typically found on the surface of B cells, monocytes and dendritic cells. CD180 is associated with a variety of biologically interesting macromolecules/ligands, for example, MD-1. CD180 is a relatively rare antibody target, with fewer than 400 publications in the last decade. Even still, CD180 is commonly used in flow cytometry applications as a phenotypic marker for differentiation of cell types, particularly in the study of immunology. This antibody was purified through affinity chromatography and conjugated to PacOrange (ex/em = 400/551 nm). It is compatible with the 405 nm laser and 525/40 nm bandpass filter (for example, as in the Beckman Coulter DxFLEX).

Spectrum


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spectrum

Spectral properties

Correction Factor (280 nm)0.6
Extinction coefficient (cm -1 M -1)25000
Excitation (nm)400
Emission (nm)551

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Conjugation


We provide custom conjugation services for this antibody (eg. labeling of cd180 antibody with HRP). A list of available labels can be found in the table below:

AFAF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
ProteinsHRP, Alkaline Phosphatase, Streptavidin
TandemsAPC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small MoleculesBiotin
Traditional DyesFITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluorUV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page here.