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PacOrange Anti-human CD19 Antibody
HIB19
HIB19 is an anti-human monoclonal antibody that recognizes the CD19 antigen. CD19 (also known as CVID3) is a 95 kD glycoprotein that is located on the surface of cells like stem cells, dendritic cells and B cells. In certain organisms, CD19 promotes release of sequestered calcium ion into cytosol, plays a role in the upregulation of phosphatidylinositol 3-kinase activity and acts to positively regulate protein kinase B signaling. In addition, it is a member of critical cellular pathways, for example, the antigen receptor-mediated signaling pathway and B cell receptor signaling pathway. From a research standpoint, it is of biological interest due to its association with key macromolecules/ligands such as lyn. CD19 is a very popular antibody target, with over 36000 publications in the last decade. CD19 is often used in flow cytometry applications as a phenotypic marker for differentiation of cell types, specifically in the study of immunology and costimulatory molecules. This antibody was purified through affinity chromatography and conjugated to PacOrange (ex/em = 400/551 nm). It is compatible with the 405 nm laser and 537/65 nm bandpass filter (for example, as in the Luminex Amnis FlowSight).
(Top) Spectral emission profiles generated using four spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm). Each laser produced a distinct emission pattern, and their combination yielded the composite spectral signature. (Bottom) Flow cytometry analysis of whole blood stained with PacOrange Anti-human CD19 Antibody (HIB19). The fluorescence signal was monitored using an Cytek Aurora flow cytometer in the specific V8-A channel.
(Top) Spectral emission profiles generated using four spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm). Each laser produced a distinct emission pattern, and their combination yielded the composite spectral signature. (Bottom) Flow cytometry analysis of whole blood stained with PacOrange Anti-human CD19 Antibody (HIB19). The fluorescence signal was monitored using an Cytek Aurora flow cytometer in the specific V8-A channel.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
101921K0100 tests
Price
101921K1500 tests
Price
 
Antibody properties
Other namesB4
CloneHIB19
Hostmouse
Reactivityhuman
ApplicationFlow Cytometry (FACS); Fluorescence Imaging
Spectral properties
Correction factor (280 nm)0.6
Extinction coefficient (cm -1 M -1)
25000
Excitation (nm)400
Emission (nm)551
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
Documents
Protocol
Safety Data Sheet
Certificate of Analysis
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Page updated on October 30, 2025