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PacOrange Anti-human CD20 Antibody
HI20a
HI20a is an anti-human monoclonal antibody that recognizes the CD20 antigen. CD20 (alternatively called MS4A1, Bp35 or Leukocyte surface antigen Leu-16) is a 33 - 37 kD transmembrane protein that is expressed on the surface of cells such as B cells. CD20 plays a role in important cellular pathways, namely, the B cell receptor signaling pathway. From a research standpoint, it is of biological interest due to its association with key macromolecules/ligands such as Lyn. CD20 is a moderately popular antibody target, with over 20000 publications in the last decade. CD20 has a variety of applications in costimulatory molecules and immunology research, commonly serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to PacOrange (ex/em = 400/551 nm). It is compatible with the 405 nm laser and 525/50 nm bandpass filter (for example, as in the Miltenyi Biotec MACSQuant VYB).
(Top) Spectral emission profiles generated using four spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm). Each laser produced a distinct emission pattern, and their combination yielded the composite spectral signature. (Bottom) Flow cytometry analysis of human whole blood stained with PacOrange Anti-human CD20 Antibody (HI20) (Cat. #102011K0). The fluorescence signal was monitored on a Cytek Aurora spectral flow cytometer in the V8-A channel, demonstrating clear detection of CD20⁺ cells.
(Top) Spectral emission profiles generated using four spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm). Each laser produced a distinct emission pattern, and their combination yielded the composite spectral signature. (Bottom) Flow cytometry analysis of human whole blood stained with PacOrange Anti-human CD20 Antibody (HI20) (Cat. #102011K0). The fluorescence signal was monitored on a Cytek Aurora spectral flow cytometer in the V8-A channel, demonstrating clear detection of CD20⁺ cells.
datasheet
Datasheet
sds
SDS
COA
CatalogSize
Price
Quantity
102011K0100 tests
Price
102011K1500 tests
Price
 
Antibody properties
Other namesBp35, B1
CloneHI20a
HostMouse
IsotypeIgG2a
ReactivityHuman
ApplicationFlow Cytometry (FACS), Fluorescence Imaging
Spectral properties
Correction factor (280 nm)0.6
Extinction coefficient (cm -1 M -1)
25000
Excitation (nm)400
Emission (nm)551
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
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Page updated on April 23, 2026