PacOrange Anti-human CD203c Antibody *NP4D6*
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Spectral properties
Correction Factor (280 nm) | 0.6 |
Extinction coefficient (cm -1 M -1) | 25000 |
Excitation (nm) | 400 |
Emission (nm) | 551 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Overview | SDSProtocol |
Other names ENPP3 | Clone NP4D6 | Host mouse | Isotype Mouse IgG1 | Reactivity human | Application Flow Cytometry (FACS); Fluorescence Imaging | Correction Factor (280 nm) 0.6 | Extinction coefficient (cm -1 M -1) 25000 | Excitation (nm) 400 | Emission (nm) 551 |
The NP4D6 monoclonal antibody binds with human CD203c, a 150 kD transmembrane protein commonly located on the surface of mast cells and basophils. In certain organisms, CD203c is an inhibitor of mast cell proliferation, is an inhibitor of mast cell activation involved in immune response and represses inflammatory response. From a research standpoint, it is of biological interest due to its association with critical macromolecules/ligands such as cAMP. CD203c is a relatively rare antibody target, with fewer than 500 publications in the last decade. Even still, CD203c is commonly used in flow cytometry applications as a phenotypic marker for differentiation of cell types, specifically in the study of immunology. This antibody was purified through affinity chromatography and conjugated to PacOrange (ex/em = 400/551 nm). It is compatible with the 405 nm laser and 530/30 nm bandpass filter (for example, as in the Agilent Technologies NovoCyte Quanteon).
Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
Correction Factor (280 nm) | 0.6 |
Extinction coefficient (cm -1 M -1) | 25000 |
Excitation (nm) | 400 |
Emission (nm) | 551 |
Antibody Finder
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Conjugation
We provide custom conjugation services for this antibody (eg. labeling of cd203c antibody with HRP). A list of available labels can be found in the table below:
For additional information about custom conjugations, please consult our services page here.
AF | AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750 |
Proteins | HRP, Alkaline Phosphatase, Streptavidin |
Tandems | APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5 |
Small Molecules | Biotin |
Traditional Dyes | FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5 |
iFluor | 350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7 |
mFluor | UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780 |
For additional information about custom conjugations, please consult our services page here.
Application notes
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Biotin Labeling Molecules and Their Biological Applications
Buccutite™ Bioconjugation Technology
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Biotin Labeling Molecules and Their Biological Applications
Buccutite™ Bioconjugation Technology
FAQ
How can I lyse my cells without lysing the nuclear membrane?
What are the differences between calcium ion indicators: Cal 520, Cal 520FF, and Cal 520N?
How do I make an AM ester stock solution?
Can we fix cells with glutaraldehyde and then stain with fluorescent phalloidin?
What is the difference between FluoroQuest Anti-fading Kit I and FluoroQuest Anti-fading Kit II?
What are the differences between calcium ion indicators: Cal 520, Cal 520FF, and Cal 520N?
How do I make an AM ester stock solution?
Can we fix cells with glutaraldehyde and then stain with fluorescent phalloidin?
What is the difference between FluoroQuest Anti-fading Kit I and FluoroQuest Anti-fading Kit II?