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PE Anti-human CD16 Antibody
3G8
The 3G8 monoclonal antibody recognizes human CD16a, a 50 - 65 kD transmembrane protein typically found on the surface of macrophages, natural killer cells, dendritic cells, monocytes and neutrophils. CD16 is a member of key cellular pathways, namely, the Fc-gamma receptor signaling pathway involved in phagocytosis. From a research standpoint, it is of biological interest due to its association with important macromolecules/ligands like IgG Fc. CD16 is a very popular antibody target, with over 25000 publications in the last decade. CD16a is frequently used in flow cytometry applications as a phenotypic marker for differentiation of cell types, particularly in the study of immunology. This antibody was purified through affinity chromatography and conjugated to PE (ex/em = 566/574 nm). It is compatible with the 561 nm laser and 585/29 nm bandpass filter (for example, as in the BD FACSJazz™).
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101611L025 tests
Price
101611L1100 tests
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101611L2500 tests
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Antibody properties

Other namesFCRIIIA
Clone3G8
HostMouse
ReactivityHuman
Spectral properties

Correction factor (280 nm)0.175
Extinction coefficient (cm -1 M -1)
1960000
Excitation (nm)565
Emission (nm)574
Quantum yield
0.82
Usage and storage

Intended useResearch Use Only (RUO)
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Page updated on August 10, 2026