PE Anti-human CD42b Antibody *HIP1*
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | N/A |
Solvent | water |
Spectral properties
Correction Factor (280 nm) | 0.175 |
Extinction coefficient (cm -1 M -1) | 1960000 |
Excitation (nm) | 565 |
Emission (nm) | 574 |
Quantum yield | 0.82 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Refrigerated (2-8 °C); Minimize light exposure |
Overview | SDSProtocol |
Other names gpIbα; Platelet glycoprotein Ib α | Clone HIP1 | Host mouse | Isotype mouse IgG1 | Reactivity human | Application | Molecular weight N/A | Correction Factor (280 nm) 0.175 | Extinction coefficient (cm -1 M -1) 1960000 | Excitation (nm) 565 | Emission (nm) 574 | Quantum yield 0.82 |
HIP1 is an anti-human monoclonal antibody that targets the CD42b antigen. CD42b (sometimes called GP1BA or Glycocalicin) is a 145 kD single-pass type i membrane protein that is found on the surface of cells such as stem cells and platelets. CD42b is involved with important cellular pathways, for instance, the cell surface receptor signaling pathway and blood coagulation, intrinsic pathway. From a research standpoint, it is of biological interest due to its association with essential macromolecules/ligands such as von Willebrand factor (vWF), Thrombin, c and d. CD42b is a relatively rare antibody target, with fewer than 800 publications in the last decade. Even still, CD42b is vital to cell adhesion and cell biology research, typically serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to PE (ex/em = 566/574 nm). It is compatible with the 561 nm laser and 585/20 nm bandpass filter (for example, as in the BD Special Order LSRFortessa™ Cell Analyzer).
Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
Correction Factor (280 nm) | 0.175 |
Extinction coefficient (cm -1 M -1) | 1960000 |
Excitation (nm) | 565 |
Emission (nm) | 574 |
Quantum yield | 0.82 |
Antibody Finder
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Conjugation
We provide custom conjugation services for this antibody (eg. labeling of cd42b antibody with HRP). A list of available labels can be found in the table below:
For additional information about custom conjugations, please consult our services page here.
AF | AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750 |
Proteins | HRP, Alkaline Phosphatase, Streptavidin |
Tandems | APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5 |
Small Molecules | Biotin |
Traditional Dyes | FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5 |
iFluor | 350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7 |
mFluor | UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780 |
For additional information about custom conjugations, please consult our services page here.
Application notes
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Biotin Labeling Molecules and Their Biological Applications
Buccutite™ Bioconjugation Technology
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Biotin Labeling Molecules and Their Biological Applications
Buccutite™ Bioconjugation Technology
FAQ
How can I lyse my cells without lysing the nuclear membrane?
What are the differences between calcium ion indicators: Cal 520, Cal 520FF, and Cal 520N?
How do I make an AM ester stock solution?
Can we fix cells with glutaraldehyde and then stain with fluorescent phalloidin?
What is the difference between FluoroQuest Anti-fading Kit I and FluoroQuest Anti-fading Kit II?
What are the differences between calcium ion indicators: Cal 520, Cal 520FF, and Cal 520N?
How do I make an AM ester stock solution?
Can we fix cells with glutaraldehyde and then stain with fluorescent phalloidin?
What is the difference between FluoroQuest Anti-fading Kit I and FluoroQuest Anti-fading Kit II?