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PE Anti-human CD50 Antibody *MEM-04*

MEM-04 is an anti-human monoclonal antibody that targets the CD50 antigen. CD50 (alternatively called ICAM-3) is a 130 kD member of the Ig superfamily that is located on the surface of cells like macrophages. CD50 is associated with a variety of biologically interesting macromolecules/ligands, in particular, integrin αd/β2 and LFA-1. CD50 is a relatively rare antibody target, with fewer than 300 publications in the last decade. Even still, CD50 is typically used in flow cytometry applications as a phenotypic marker for differentiation of cell types, particularly in the study of costimulatory molecules and cell biology. This antibody was purified through affinity chromatography and conjugated to PE (ex/em = 566/574 nm). It is compatible with the 561 nm laser and 586/15 nm bandpass filter (for example, as in the BD FACSCelesta™).

Spectrum

Conjugation

We provide custom conjugation services for this antibody (eg. labeling of cd50 antibody with HRP). A list of available labels can be found in the table below:

AFAF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
ProteinsHRP, Alkaline Phosphatase, Streptavidin
TandemsAPC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small MoleculesBiotin
Traditional DyesFITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluorUV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page here.
Page updated on May 13, 2025

Ordering information

Price
Unit size
25 tests
100 tests
500 tests
Catalog Number
105001M0105001M1105001M2
Quantity
Add to cart

Additional ordering information

Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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Technical SupportContact us
Purchase orderSend to sales@aatbio.com
ShippingStandard overnight for United States, inquire for international
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Antibody properties

Other namesICAM-3
CloneMEM-04
Hostmouse
IsotypeMouse IgG2b kappa
Reactivityhuman
Application

Spectral properties

Correction Factor (280 nm)

0.175

Extinction coefficient (cm -1 M -1)

1960000

Excitation (nm)

565

Emission (nm)

574

Quantum yield

0.82

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
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