PE/Cy5 Anti-dog/ chicken/ rabbit/ guinea pig/ horse/ cow/ mouse/ rat/ pig/ non-human primates/ human CD79a Antibody HM47

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	1960000
Excitation (nm)	565
Emission (nm)	666

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22

Overview SDS Protocol

Other names

Mb-1; IGA

Clone

HM47

Host

mouse

Isotype

Mouse IgG1 kappa

Reactivity

dog; chicken; rabbit; guinea pig; horse; cow; mouse; rat; pig; non-human primates; human

Application

Extinction coefficient (cm ^-1 M ^-1)

1960000

Excitation (nm)

565

Emission (nm)

666

HM47 is an anti-dog/ chicken/ rabbit/ guinea pig/ horse/ cow/ mouse/ rat/ pig/ non-human primates/ human monoclonal antibody that forms an immune complex with the CD79a antigen. CD79a (sometimes referred to as Mb-1 or IGA) is a 47 kD single-pass type I membrane protein that is expressed on the surface of cells such as B cells. CD79a is a component of essential cellular pathways, in particular, the B cell receptor signaling pathway. From a research standpoint, it is of biological interest due to its association with key macromolecules/ligands like CD22, CD79b, CD5 and CD19. CD79a is a fairly uncommon antibody target, with a little more than 4100 publications in the last decade. Even still, CD79a has been widely used in immunology research, commonly serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to PE/Cy5 (ex/em = 565/666 nm). It is compatible with the 561 nm laser and 697/58 nm bandpass filter (for example, as in the BD FACSMelody™).

Spectrum

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Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	1960000
Excitation (nm)	565
Emission (nm)	666

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Conjugation

We provide custom conjugation services for this antibody (eg. labeling of cd79a antibody with HRP). A list of available labels can be found in the table below:

AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780