Name: PE/Cy7 Anti-human CD19 Antibody *HI19a*
Brand: AAT Bioquest
SKU: 101901N0
Availability: InStock

PE/Cy7 Anti-human CD19 Antibody

HI19a

PE/Cy7 Anti-Human CD19 Antibody is a fluorescent anti-human monoclonal antibody (clone HI19a) that targets CD19 for flow cytometric identification of B-lineage and CD19-positive cell populations.

CD19-specific clone: Uses the HI19a monoclonal antibody clone to target human CD19, a 95 kD transmembrane glycoprotein expressed on B cells and related cell populations
PE/Cy7 tandem fluorescence: Conjugated to PE/Cy7 with excitation/emission maxima of approximately 566/778 nm
Flow cytometry compatibility: Compatible with 561 nm laser excitation and 780/60 nm bandpass filter detection
Affinity-purified format: Purified by affinity chromatography before PE/Cy7 conjugation
Research applications: Suitable for flow cytometric immunophenotyping of human B cells and CD19-positive cell populations

(Top) Spectral emission profiles generated using four spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm). Each laser produced a distinct emission pattern, and their combination yielded the composite spectral signature of PE/Cy7. (Bottom) Flow cytometry analysis of whole blood stained with PE/Cy7 anti-human CD19 antibody (Cat. #101901N0). The fluorescence signal was monitored using a Cytek Aurora spectral flow cytometer in the B14-A channel.

Datasheet

SDS

COA

Catalog	Size	Price
101901N0	25 tests	Price
101901N1	100 tests	Price
101901N2	500 tests	Price

Overview

PE/Cy7 Anti-Human CD19 Antibody is an affinity-purified monoclonal antibody conjugate using the HI19a clone, which targets the human CD19 antigen. CD19, also known as T-cell surface antigen Leu-12 or B-lymphocyte surface antigen B4, is a 95 kD transmembrane glycoprotein found on the surface of B cells and other CD19-expressing cell populations. CD19 is involved in antigen receptor-mediated signaling and B cell receptor signaling pathways and is biologically associated with molecules such as CD81 and Leu-13.

This antibody is conjugated to PE/Cy7, a tandem fluorophore with excitation/emission maxima of approximately 566/778 nm. The conjugate is compatible with 561 nm laser excitation and 780/60 nm bandpass filter detection, including configurations used on instruments such as the Agilent Technologies NovoCyte Quanteon. PE/Cy7 Anti-Human CD19 Antibody is suitable for flow cytometry-based immunophenotyping, B cell analysis, immunology research, and detection of human CD19-positive cell populations.

Conjugation

We provide custom conjugation services for this antibody (e.g. labeling of CD19 antibody with HRP). A list of available labels can be found in the table below:

Label Type	Available Labels
AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page

here

References

View all 50 references: Citation Explorer

Implementation of a one-tube flow cytometry panel for measurable residual disease detection in multiple myeloma patients in clinical routine.

Authors:

Deutsch-Biedermann, Karin and Hefler-Frischmuth, Katrin and Gruber, Judith and Kimbacher, Christine and Herbring, Isabella and Machherndl-Spandl, Sigrid and Strassl, Irene and Bettelheim, Peter and Dieplinger, Benjamin

Journal:

Cytometry. Part B, Clinical cytometry (2026): 22-28

Utility of multiparametric flow cytometry in the diagnosis and staging of Classic Hodgkin lymphoma-Experience in real-world practice.

Authors:

Balaji, K and Ghogale, Sitaram G and Deshpande, Nilesh and Patil, Jagruti and Girase, Karishma and Poojary, Suresh and Chatterjee, Gaurav and Rajpal, Sweta and Patkar, Nikhil and Epari, Sridhar and Shet, Tanuja and Gujral, Sumeet and Subramanian, P G and Tembhare, Prashant R

Journal:

Cytometry. Part B, Clinical cytometry (2026)

Development and computational analysis of high dimensional spectral flow cytometry data for the resolution of innate lymphoid cells in the mammary tumor microenvironment.

Authors:

Seo, Hobin and Xue, Jingna and Huang, Qiutong and Kinzel, Megan and Verma, Amisha and Huynh, Ngan and Jamila Ikra, Zahra and Mahoney, Douglas J and Lee, Jongbok and Morrissy, Sorana and Jacquelot, Nicolas

Journal:

Frontiers in immunology (2026): 1730567

Clinical implementation of a one-step no-wash flow cytometry method allows for real-time monitoring of patients treated with autologous CAR-T cells.

Authors:

Salem, Nassim and Demerlé, Clémence and Mfarrej, Bechara and Blaison, Ludovic and Lignée, Pierre and Couquiaud, Jérôme and Ouffai, Sarah and Malenfant, Carine and Reichert, Thibaut and Demaret, Julie and Trauet, Jacques and Yakoub-Agha, Ibrahim and Legrand Izadifar, Faezeh and Granata, Angela and Thévenet, Sophie and Château, Anne-Line and Calmels, Boris and Chabannon, Christian and Brisou, Gabriel and Lemarié, Claude

Journal:

Frontiers in oncology (2026): 1774431

Prognostic value of flow cytometry in myelodysplastic neoplasms (MDS): Composition of a FCM-prognostic score (FCM-PS) for overall survival.

Authors:

Santaolalla, Aida and Oelschlaegel, Uta and Winter, Susann and Jamshidi, Shirin and Westers, Theresia M and Sockel, Katja and Bornhäuser, Martin and Ejarque, Rosa Andres and Farzaneh, Farzin and Poloni, Antonella and Kubasch, Anne-Sophie and Van Hemelrijck, Mieke and van de Loosdrecht, Arjan A and Platzbecker, Uwe and Kordasti, Shahram

Journal:

HemaSphere (2026): e70293

Antibody properties

Other names	B4
Clone	HI19a
Host	Mouse
Isotype	IgG1
Reactivity	Human

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	1960000
Excitation (nm)	565
Emission (nm)	778

Usage and storage

Intended use

Research Use Only (RUO)

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on November 7, 2026