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PE/Cy7 Anti-human CD28 Antibody
CD28.2
CD28.2 is an anti-human monoclonal antibody that forms an immune complex with the CD28 antigen. CD28 (sometimes called T44) is a 44 kD member of the Ig superfamily that is located on the surface of cells such as T cells. CD28 acts in essential cellular pathways, in particular, the cell surface receptor signaling pathway, T cell receptor signaling pathway and apoptotic signaling pathway. Furthermore, in some organisms, it positively regulates isotype switching to IgG isotypes, is an enhancer of interleukin-4 production and upregulates translation. From a research standpoint, it is of biological interest due to its association with vital macromolecules/ligands such as PI3-kinase, CD80 and CD86. CD28 is a very popular antibody target, with over 30000 publications in the last decade. CD28 is essential for costimulatory molecules and immunology research, typically serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to PE/Cy7 (ex/em = 566/778 nm). It is compatible with the 561 nm laser and 780/60 nm bandpass filter (for example, as in the BD FACSAria™ Fusion).
(Top) Spectral emission profiles generated using four spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm). Each laser produced a distinct emission pattern, and their combination yielded the composite spectral signature. (Bottom) Flow cytometry analysis of human whole blood stained with PE/Cy7 Anti-human CD28 Antibody (CD28.2) (Cat. #102801N1). The fluorescence signal was monitored on a Cytek Aurora spectral flow cytometer in the B14-A channel, demonstrating clear detection of CD28⁺ cells.
(Top) Spectral emission profiles generated using four spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm). Each laser produced a distinct emission pattern, and their combination yielded the composite spectral signature. (Bottom) Flow cytometry analysis of human whole blood stained with PE/Cy7 Anti-human CD28 Antibody (CD28.2) (Cat. #102801N1). The fluorescence signal was monitored on a Cytek Aurora spectral flow cytometer in the B14-A channel, demonstrating clear detection of CD28⁺ cells.
datasheet
Datasheet
sds
SDS
COA
CatalogSize
Price
Quantity
102801N025 tests
Price
102801N1100 tests
Price
102801N2500 tests
Price
 
Antibody properties
Other namesTp44, T44
CloneCD28.2
HostMouse
ReactivityHuman
Spectral properties
Extinction coefficient (cm -1 M -1)
1960000
Excitation (nm)565
Emission (nm)778
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
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Page updated on May 16, 2026