Name: PE/iFluor® 594 Anti-human CD148 Antibody *MEM-CD148/05*
Brand: AAT Bioquest
SKU: 114801Y0
Availability: InStock

PE/iFluor® 594 Anti-human CD148 Antibody

MEM-CD148/05

MEM-CD148/05 is an anti-human monoclonal antibody that forms an immune complex with the CD148 antigen. CD148 (sometimes called p260 or DEP-1) is a glycoprotein that is found on the surface of cells such as dendritic cells, platelets, B cells and NK cells. CD148 acts in key cellular pathways, for instance, the T cell receptor signaling pathway, negative regulation of epidermal growth factor receptor signaling pathway and positive regulation of Fc-gamma receptor signaling pathway involved in phagocytosis. In addition, in many organisms, it upregulates MAPK cascade, is an inhibitor of cell growth and represses cell migration. From a research standpoint, it is of biological interest due to its association with vital macromolecules/ligands. CD148 is a relatively rare antibody target, with fewer than 500 publications in the last decade. Even still, CD148 is typically used in flow cytometry applications as a phenotypic marker for differentiation of cell types, specifically in the study of immunology. This antibody was purified through affinity chromatography and conjugated to PE/iFluor® 594 (ex/em = 566/606 nm). It is compatible with the 561 nm laser and 620/15 nm bandpass filter (for example, as in the Thermo Fisher Attune NxT).

Protocol

COA

Catalog	Size	Price
114801Y0	25 tests	Price
114801Y1	100 tests	Price
114801Y2	500 tests	Price

Conjugation

We provide custom conjugation services for this antibody (e.g. labeling of cd148 antibody with HRP). A list of available labels can be found in the table below:

Label Type	Available Labels
AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page

here

Antibody properties

Other names	DEP-1; p260
Clone	MEM-CD148/05
Host	mouse
Isotype	Mouse IgG2b
Reactivity	human

Spectral properties

Absorbance (nm)	566
Extinction coefficient (cm ^-1 M ^-1)	1960000
Excitation (nm)	565
Emission (nm)	606

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 5, 2025