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PE/iFluor® 594 Anti-human CD3 Antibody
OKT-3
OKT-3 is an anti-human monoclonal antibody that targets the CD3e antigen. CD3e (alternatively called T cell antigen receptor complex or TCRE) is a 20 kD member of the Ig superfamily that is located on the surface of cells like T cells. CD3 is a member of important cellular pathways, in particular, the cell surface receptor signaling pathway, T cell receptor signaling pathway and negative regulation of smoothened signaling pathway. Furthermore, in certain organisms, it positively regulates calcium-mediated signaling, upregulates peptidyl-tyrosine phosphorylation and enhances cell-matrix adhesion. From a research standpoint, it is of biological interest due to its association with vital macromolecules/ligands such as TCR. CD3 is a very popular antibody target, with over 80000 publications in the last decade. CD3e is vital to immunology research, typically serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to PE/iFluor® 594 (ex/em = 566/606 nm). It is compatible with the 561 nm laser and 610/20 nm bandpass filter (for example, as in the BD LSRFortessa™ X-20).
(Top) Spectral emission profiles generated using four spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm). Each laser produced a distinct emission pattern, and their combination yielded the composite spectral signature. (Bottom) Flow cytometry analysis of whole blood stained with PE/iFluor® 594 Anti-human CD3 Antibody *OKT-3*. The fluorescence signal was monitored using an Cytek Aurora flow cytometer in the specific B6-A channel.
(Top) Spectral emission profiles generated using four spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm). Each laser produced a distinct emission pattern, and their combination yielded the composite spectral signature. (Bottom) Flow cytometry analysis of whole blood stained with PE/iFluor® 594 Anti-human CD3 Antibody *OKT-3*. The fluorescence signal was monitored using an Cytek Aurora flow cytometer in the specific B6-A channel.
protocol
Protocol
COA
CatalogSize
Price
Quantity
100341Y025 tests
Price
100341Y1100 tests
Price
100341Y2500 tests
Price
 
Antibody properties
Other namesT3E
CloneOKT-3
Hostmouse
Isotypemouse igg2a, κ
Reactivityhuman
Spectral properties
Absorbance (nm)566
Extinction coefficient (cm -1 M -1)
1960000
Excitation (nm)565
Emission (nm)606
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
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Page updated on October 6, 2025