Name: PE/iFluor® 594 Anti-human CD33 Antibody *HI33a*
Brand: AAT Bioquest
SKU: 103301Y0
Availability: InStock

PE/iFluor® 594 Anti-human CD33 Antibody

HI33a

The HI33a monoclonal antibody recognizes human CD33, a 67 kD single-pass type i membrane protein frequently expressed on the surface of monocytes, neutrophils, dendritic cells and mast cells. In some organisms, CD33 plays a role in the downregulation of calcium ion transport, is a suppressor of tumor necrosis factor production and is an inhibitor of interleukin-1 beta production. Additionally, it has been closely linked to vital biological processes such as signal transduction, especially immune response-inhibiting signal transduction. From a research standpoint, it is of biological interest due to its association with important macromolecules/ligands such as α-2 and 6- linked Sialic acid. CD33 is a fairly uncommon antibody target, with a little more than 8000 publications in the last decade. Even still, CD33 is typically used in flow cytometry applications as a phenotypic marker for differentiation of cell types, specifically in the study of neuroscience. This antibody was purified through affinity chromatography and conjugated to PE/iFluor® 594 (ex/em = 566/606 nm). It is compatible with the 561 nm laser and 610/20 nm bandpass filter (for example, as in the BD FACSAria™ III).

Protocol

COA

Catalog	Size	Price
103301Y0	25 tests	Price
103301Y1	100 tests	Price
103301Y2	500 tests	Price

Conjugation

We provide custom conjugation services for this antibody (e.g. labeling of cd33 antibody with HRP). A list of available labels can be found in the table below:

Label Type	Available Labels
AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page

here

Antibody properties

Other names	Siglec-3; gp67
Clone	HI33a
Host	mouse
Isotype	mouse IgG2a
Reactivity	human

Spectral properties

Absorbance (nm)	566
Extinction coefficient (cm ^-1 M ^-1)	1960000
Excitation (nm)	565
Emission (nm)	606

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 9, 2025