Name: PE/iFluor® 594 Anti-human CD47 Antibody *HI172*
Brand: AAT Bioquest
SKU: 104711Y0
Availability: InStock

PE/iFluor® 594 Anti-human CD47 Antibody

HI172

HI172 is an anti-human monoclonal antibody that is specific for the CD47 antigen. CD47 (sometimes called Integrin associated protein) is a 42 - 52 kD multi-pass membrane protein that is found on the surface of cells such as granulocytes, platelets, endothelial cells and B cells. CD47 acts in critical cellular pathways, in particular, the negative regulation of Fc-gamma receptor signaling pathway involved in phagocytosis and integrin-mediated signaling pathway. In addition, in certain organisms, it downregulates Fc-gamma receptor signaling pathway involved in phagocytosis, upregulates T cell activation and acts to positively regulate inflammatory response. From a research standpoint, it is of biological interest due to its association with key macromolecules/ligands like SIRP, Thrombospondin and CD61. CD47 is a fairly uncommon antibody target, with a little more than 5000 publications in the last decade. Even still, CD47 is often used in flow cytometry applications as a phenotypic marker for differentiation of cell types, particularly in the study of immunology. This antibody was purified through affinity chromatography and conjugated to PE/iFluor® 594 (ex/em = 566/606 nm). It is compatible with the 561 nm laser and 613/18 nm bandpass filter (for example, as in the BD FACSMelody™).

Protocol

COA

Catalog	Size	Price
104711Y0	25 tests	Price
104711Y1	100 tests	Price
104711Y2	500 tests	Price

Conjugation

We provide custom conjugation services for this antibody (e.g. labeling of cd47 antibody with HRP). A list of available labels can be found in the table below:

Label Type	Available Labels
AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page

here

Antibody properties

Other names	gp42; IAP; neurophilin; MER6; Integrin associated protein
Clone	HI172
Host	mouse
Isotype	mouse IgG1
Reactivity	human

Spectral properties

Absorbance (nm)	566
Extinction coefficient (cm ^-1 M ^-1)	1960000
Excitation (nm)	565
Emission (nm)	606

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 8, 2025