Name: PE/iFluor® 594 Anti-human CD58 Antibody *HI58a*
Brand: AAT Bioquest
SKU: 105801Y0
Availability: InStock

PE/iFluor® 594 Anti-human CD58 Antibody

HI58a

HI58a is an anti-human monoclonal antibody that targets the CD58 antigen. CD58 (sometimes referred to as Lymphocyte function-associated antigen 3, LFA3 or LFA-3) is a 45 - 70 kD transmembrane protein that is located on the surface of cells such as macrophages, NK cells, dendritic cells, B cells and granulocytes. CD58 has been associated with key biological processes such as cell-cell adhesion, especially heterotypic cell-cell adhesion. Also, in certain organisms, it is involved in the positive regulation of interleukin-8 secretion. From a research standpoint, it is of biological interest due to its association with important macromolecules/ligands like CD2 and LFA-2. CD58 is a relatively rare antibody target, with less than 1000 publications in the last decade. Even still, CD58 is commonly used in flow cytometry applications as a phenotypic marker for differentiation of cell types, especially in the study of cell biology. This antibody was purified through affinity chromatography and conjugated to PE/iFluor® 594 (ex/em = 566/606 nm). It is compatible with the 561 nm laser and 589/15 nm bandpass filter (for example, as in the Bio-Rad ZE5 Cell Analyzer).

Protocol

COA

Catalog	Size	Price
105801Y0	25 tests	Price
105801Y1	100 tests	Price
105801Y2	500 tests	Price

Conjugation

We provide custom conjugation services for this antibody (e.g. labeling of cd58 antibody with HRP). A list of available labels can be found in the table below:

Label Type	Available Labels
AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page

here

Antibody properties

Other names	LFA-3
Clone	HI58a
Host	mouse
Isotype	mouse IgG1
Reactivity	human

Spectral properties

Absorbance (nm)	566
Extinction coefficient (cm ^-1 M ^-1)	1960000
Excitation (nm)	565
Emission (nm)	606

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 6, 2025