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PE/iFluor® 594 Anti-human CD7 Antibody
HIT7
HIT7 is an anti-human monoclonal antibody that recognizes the CD7 antigen. CD7 (also known as gp40) is a 40 kD single-pass type I membrane protein that is located on the surface of cells like NK cells, stem cells and T cells. CD7 has been closely linked to essential biological processes such as immune response, particularly adaptive immune response. Furthermore, it is involved with important cellular pathways, namely, the transmembrane receptor protein tyrosine kinase signaling pathway. From a research standpoint, it is of biological interest due to its association with vital macromolecules/ligands like PI3-Kinase. CD7 is a fairly uncommon antibody target, with a little more than 3600 publications in the last decade. Even still, CD7 is commonly used in flow cytometry applications as a phenotypic marker for differentiation of cell types, especially in the study of immunology and costimulatory molecules. This antibody was purified through affinity chromatography and conjugated to PE/iFluor® 594 (ex/em = 566/606 nm). It is compatible with the 561 nm laser and 615/20 nm bandpass filter (for example, as in the Miltenyi Biotec MACSQuant VYB).
(Top) Spectral emission profiles generated using four spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm). Each laser produced a distinct emission pattern, and their combination yielded the composite spectral signature. (Bottom) Flow cytometry analysis of whole blood stained with PE/iFluor® 594 Anti-human CD7 Antibody *HIT7*. The fluorescence signal was monitored using an Cytek Aurora flow cytometer in the specific B6-A channel.
(Top) Spectral emission profiles generated using four spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm). Each laser produced a distinct emission pattern, and their combination yielded the composite spectral signature. (Bottom) Flow cytometry analysis of whole blood stained with PE/iFluor® 594 Anti-human CD7 Antibody *HIT7*. The fluorescence signal was monitored using an Cytek Aurora flow cytometer in the specific B6-A channel.
protocol
Protocol
COA
CatalogSize
Price
Quantity
100701Y025 tests
Price
100701Y1100 tests
Price
100701Y2500 tests
Price
 
Antibody properties
Other namesgp40; TP41
CloneHIT7
Hostmouse
Isotypemouse IgG1
Reactivityhuman
Spectral properties
Absorbance (nm)566
Extinction coefficient (cm -1 M -1)
1960000
Excitation (nm)565
Emission (nm)606
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
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Page updated on October 10, 2025