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PE/iFluor® 594 Anti-human CD8 Antibody
SK1
SK1 is an anti-human monoclonal antibody that is specific for the CD8a antigen. CD8a (sometimes called MAL or Leu2) is a 32 - 34 kD member of the Ig superfamily that is expressed on the surface of cells such as NK cells and T cells. CD8 is a component of important cellular pathways, for example, the transmembrane receptor protein tyrosine kinase signaling pathway and cell surface receptor signaling pathway. From a research standpoint, it is of biological interest due to its association with important macromolecules/ligands like MHCI. CD8 is a very popular antibody target, with over 125000 publications in the last decade. CD8a is commonly used in flow cytometry applications as a phenotypic marker for differentiation of cell types, especially in the study of immunology. This antibody was purified through affinity chromatography and conjugated to PE/iFluor® 594 (ex/em = 566/606 nm). It is compatible with the 561 nm laser and 610/20 nm bandpass filter (for example, as in the BD FACSAria™ III).
(Top) Spectral emission profiles generated using four spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm). Each laser produced a distinct emission pattern, and their combination yielded the composite spectral signature. (Bottom) Flow cytometry analysis of whole blood stained with PE/iFluor® 594 Anti-human CD8 Antibody *SK1*. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the  specific B6-A channel.
(Top) Spectral emission profiles generated using four spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm). Each laser produced a distinct emission pattern, and their combination yielded the composite spectral signature. (Bottom) Flow cytometry analysis of whole blood stained with PE/iFluor® 594 Anti-human CD8 Antibody *SK1*. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the  specific B6-A channel.
protocol
Protocol
COA
CatalogSize
Price
Quantity
100811Y025 tests
Price
100811Y1100 tests
Price
100811Y2500 tests
Price
 
Physical properties
Molecular weightN/A
Solventwater
Antibody properties
Other namesT8; Leu2
CloneSK1
Hostmouse
Reactivityhuman
Spectral properties
Absorbance (nm)566
Extinction coefficient (cm -1 M -1)
1960000
Excitation (nm)565
Emission (nm)606
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageRefrigerated (2-8 °C); Minimize light exposure
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Page updated on October 5, 2025