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PE/iFluor® 700 Anti-human CD25 Antibody
HI25a
HI25a is an anti-human monoclonal antibody that is specific for the CD25 antigen. CD25 (sometimes called IL2RA) is a 55 kD transmembrane protein that is found on the surface of cells such as T cells, macrophages, B cells and NK cells. In certain organisms, CD25 is involved in the positive regulation of activated T cell proliferation, is a negative regulator of T cell proliferation and plays a role in the upregulation of T cell differentiation. Additionally, it is a component of important cellular pathways, for example, the cytokine-mediated signaling pathway, interleukin-2-mediated signaling pathway and Notch signaling pathway. From a research standpoint, it is of biological interest due to its association with key macromolecules/ligands like IL-2. CD25 is a very popular antibody target, with over 40000 publications in the last decade. CD25 has a variety of applications in immunology research, frequently serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to PE/iFluor® 700 (ex/em = 566/708 nm). It is compatible with the 561 nm laser and 702/85 nm bandpass filter (for example, as in the Luminex Amnis FlowSight).
| Catalog | Size | Price | Quantity |
|---|---|---|---|
| 102501X0 | 25 tests | Price | |
| 102501X1 | 100 tests | Price | |
| 102501X2 | 500 tests | Price |
Physical properties
| Molecular weight | N/A |
| Solvent | water |
Antibody properties
| Other names | IL-2Rα; p55; TAC antigen |
| Clone | HI25a |
| Host | mouse |
| Isotype | mouse IgG1 |
| Reactivity | human |
Spectral properties
| Absorbance (nm) | 566 |
| Extinction coefficient (cm -1 M -1) | 1960000 |
| Excitation (nm) | 565 |
| Emission (nm) | 708 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Refrigerated (2-8 °C); Minimize light exposure |
Contact us
| Telephone | |
| Fax | |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Technical Support | Contact us |
| Request quotation | Request |
| Purchase order | Send to sales@aatbio.com |
| Shipping | Standard overnight for United States, inquire for international |
Page updated on October 9, 2025