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PE/iFluor® 700 Anti-human CD4 Antibody
HIT4a
HIT4a is an anti-human monoclonal antibody that targets the CD4 antigen. CD4 (alternatively called Leu3a or T4) is a 55 kD single-pass type I membrane protein that is expressed on the surface of cells such as granulocytes, T cells and macrophages. CD4 is involved with key cellular pathways, namely, the enzyme linked receptor protein signaling pathway, transmembrane receptor protein tyrosine kinase signaling pathway and cytokine-mediated signaling pathway. Moreover, in many organisms, it is involved in the positive regulation of monocyte differentiation, upregulates MAPK cascade and is an enhancer of ERK1 and ERK2 cascade. CD4 has been associated with vital biological processes like immune response, particularly adaptive immune response, and is associated with a variety of biologically interesting macromolecules/ligands, for example, Lck, IL-16, MHC Class II and gp120. CD4 is a very popular antibody target, with over 180000 publications in the last decade. CD4 is essential for immunology research, typically serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to PE/iFluor® 700 (ex/em = 566/708 nm). It is compatible with the 561 nm laser and 695/40 nm bandpass filter (for example, as in the Agilent Technologies NovoCyte Quanteon).
| Catalog | Size | Price | Quantity |
|---|---|---|---|
| 100401X0 | 25 tests | Price | |
| 100401X1 | 100 tests | Price | |
| 100401X2 | 500 tests | Price |
Antibody properties
| Other names | Leu-3; T4 |
| Clone | HIT4a |
| Host | mouse |
| Isotype | mouse IgG2b |
| Reactivity | human |
Spectral properties
| Absorbance (nm) | 566 |
| Extinction coefficient (cm -1 M -1) | 1960000 |
| Excitation (nm) | 565 |
| Emission (nm) | 708 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
Contact us
| Telephone | |
| Fax | |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Technical Support | Contact us |
| Request quotation | Request |
| Purchase order | Send to sales@aatbio.com |
| Shipping | Standard overnight for United States, inquire for international |
Page updated on October 10, 2025