Name: PE/iFluor® 700 Anti-human CD45 Antibody *HI151*
Brand: AAT Bioquest
SKU: 104521X0
Availability: InStock

PE/iFluor® 700 Anti-human CD45 Antibody

HI151

HI151 is an anti-human monoclonal antibody that targets the CD45 antigen. CD45 (also known as Leukocyte Common Antigen (LCA) or PTPRC) is a 180 - 240 kD transmembrane protein that is expressed on the surface of cells like B cells, macrophages, granulocytes and NK cells. CD45 has been associated with essential biological processes like dephosphorylation, specifically protein dephosphorylation. Also, in some organisms, it plays a role in the upregulation of stem cell proliferation, is an inhibitor of cytokine-mediated signaling pathway and enhances hematopoietic stem cell migration. CD45 acts in vital cellular pathways, for example, the B cell receptor signaling pathway, positive regulation of antigen receptor-mediated signaling pathway and negative regulation of cytokine-mediated signaling pathway. From a research standpoint, it is of biological interest due to its association with important macromolecules/ligands like Src kinases. CD45 is a very popular antibody target, with over 50000 publications in the last decade. CD45 is frequently used in flow cytometry applications as a phenotypic marker for differentiation of cell types, particularly in the study of cell biology and neuroscience. This antibody was purified through affinity chromatography and conjugated to PE/iFluor® 700 (ex/em = 566/708 nm). It is compatible with the 561 nm laser and 710/50 nm bandpass filter (for example, as in the BD LSRFortessa™ X-20).

Protocol

COA

Catalog	Size	Price
104521X0	25 tests	Price
104521X1	100 tests	Price
104521X2	500 tests	Price

Conjugation

We provide custom conjugation services for this antibody (e.g. labeling of cd45 antibody with HRP). A list of available labels can be found in the table below:

Label Type	Available Labels
AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page

here

Antibody properties

Other names	Leukocyte Common Antigen (LCA); T200; PTPRC
Clone	HI151
Host	mouse
Isotype	mouse IgG1
Reactivity	human

Spectral properties

Absorbance (nm)	566
Extinction coefficient (cm ^-1 M ^-1)	1960000
Excitation (nm)	565
Emission (nm)	708

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 8, 2025