Name: PE/iFluor® 750 Anti-human CD19 Antibody *HI19a*
Brand: AAT Bioquest
SKU: 101901R0
Availability: InStock

PE/iFluor® 750 Anti-human CD19 Antibody

HI19a

Mouse Anti-Human CD19 Antibody, PE/iFluor® 750-Labeled is a fluorescent monoclonal antibody clone HI19a that targets human CD19 for flow cytometric identification of B-lineage cells.

CD19-specific clone: Uses the HI19a monoclonal antibody clone to target human CD19, a transmembrane glycoprotein expressed on B-lineage cells
PE/iFluor® 750 tandem fluorescence: Conjugated to PE/iFluor® 750 with excitation/emission maxima of approximately 566/778 nm
561 nm laser compatibility: Suitable for flow cytometry detection using 561 nm laser excitation and far-red emission collection
Affinity-purified format: Purified by affinity chromatography before fluorescent dye conjugation
Research applications: Suitable for flow cytometric immunophenotyping of human B cells and CD19-positive cell populations

(Top) Spectral emission profiles generated using four spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm). Each laser produced a distinct emission pattern, and their combination yielded the composite spectral signature of PE/iFluor® 750.(Bottom) Flow cytometry analysis of whole blood stained with PE/iFluor® 750 anti-human CD19 antibody (Cat. #101901R0). The fluorescence signal was monitored using a Cytek Aurora spectral flow cytometer in the B14-A channel.

Datasheet

SDS

COA

Catalog	Size	Price
101901R0	25 tests	Price
101901R1	100 tests	Price
101901R2	500 tests	Price

Overview

Mouse Anti-Human CD19 Antibody, PE/iFluor® 750-Labeled is an affinity-purified monoclonal antibody conjugate using the HI19a clone, which targets human CD19. CD19, also known as T-cell surface antigen Leu-12 or B-lymphocyte surface antigen B4, is a transmembrane glycoprotein widely used as a phenotypic marker for B-lineage cells. CD19 is involved in B cell receptor-associated signaling pathways and is biologically associated with molecules such as CD81 and Leu-13.

This antibody is conjugated to PE/iFluor® 750, a tandem fluorophore with excitation/emission maxima of approximately 566/778 nm. The conjugate is compatible with 561 nm laser excitation and far-red detection, including 762/35 nm bandpass filter configurations such as those used on instruments like the Luminex Amnis FlowSight. Mouse Anti-Human CD19 Antibody, PE/iFluor® 750-Labeled is suitable for flow cytometry-based immunophenotyping, B cell analysis, and detection of CD19-positive human cell populations.

Conjugation

We provide custom conjugation services for this antibody (e.g. labeling of CD19 antibody with HRP). A list of available labels can be found in the table below:

Label Type	Available Labels
AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page

here

Antibody properties

Other names	B4
Clone	HI19a
Host	Mouse
Isotype	IgG1
Reactivity	Human

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	1960000
Excitation (nm)	565
Emission (nm)	778

Usage and storage

Intended use

Research Use Only (RUO)

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Page updated on November 10, 2026