PE/XFD700 Anti-human CD20 Antibody *HI47*

HI47 is an anti-human monoclonal antibody that recognizes the CD20 antigen. CD20 (alternatively called Leukocyte surface antigen Leu-16) is a 33 - 37 kD transmembrane protein that is located on the surface of cells such as B cells and T cells. CD20 is a member of critical cellular pathways, in particular, the B cell receptor signaling pathway. From a research standpoint, it is of biological interest due to its association with essential macromolecules/ligands like lyn. CD20 is a very popular antibody target, with over 28000 publications in the last decade. CD20 has been widely used in immunology and costimulatory molecules research, often serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to PE/XFD700 (ex/em = 566/721 nm). XFD700 is manufactured by AAT Bioquest, and it has a chemical structure similar to that of Alexa Fluor® 700 (Alexa Fluor® is the trademark of Thermo Fisher). It is compatible with the 561 nm laser and 720/60 nm bandpass filter (for example, as in the Bio-Rad ZE5 Cell Analyzer).

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