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PE/XFD700 Anti-human CD27 Antibody
O323
O323 is an anti-human monoclonal antibody that is specific for the CD27 antigen. CD27 (also known as S152, TNFRSF7 or T14) is a 50 - 55 kD single-pass type I membrane protein that is found on the surface of cells such as T cells, B cells and NK cells. In some organisms, CD27 promotes B cell differentiation, inhibits apoptotic process and positively regulates NIK/NF-kappaB signaling. Additionally, it is a member of vital cellular pathways, for instance, the extrinsic apoptotic signaling pathway, cell surface receptor signaling pathway and tumor necrosis factor-mediated signaling pathway. From a research standpoint, it is of biological interest due to its association with key macromolecules/ligands such as CD70. CD27 is a moderately popular antibody target, with over 11000 publications in the last decade. CD27 is vital to costimulatory molecules research, typically serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to PE/XFD700 (ex/em = 566/721 nm). XFD700 is manufactured by AAT Bioquest, and it has a chemical structure similar to that of Alexa Fluor® 700 (Alexa Fluor® is the trademark of Thermo Fisher). It is compatible with the 561 nm laser and 710/50 nm bandpass filter (for example, as in the BD Special Order LSRFortessa™ Cell Analyzer).
(Top) Spectral emission profiles generated using four spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm). Each laser produced a distinct emission pattern, and their combination yielded the composite spectral signature. (Bottom) Flow cytometry analysis of human whole blood stained with PE/XFD700 Anti-human CD27 Antibody (O323) (Cat. #102711O1). The fluorescence signal was monitored on a Cytek Aurora spectral flow cytometer in the B10-A channel, demonstrating clear detection of CD27⁺ cells.
(Top) Spectral emission profiles generated using four spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm). Each laser produced a distinct emission pattern, and their combination yielded the composite spectral signature. (Bottom) Flow cytometry analysis of human whole blood stained with PE/XFD700 Anti-human CD27 Antibody (O323) (Cat. #102711O1). The fluorescence signal was monitored on a Cytek Aurora spectral flow cytometer in the B10-A channel, demonstrating clear detection of CD27⁺ cells.
datasheet
Datasheet
COA
CatalogSize
Price
Quantity
102711O025 tests
Price
102711O1100 tests
Price
102711O2500 tests
Price
 
Physical properties
Molecular weightN/A
Solventwater
Antibody properties
Other namesT14, S152, TNFRSF7
CloneO323
HostMouse
IsotypeIgG1, kappa
ReactivityHuman
Spectral properties
Extinction coefficient (cm -1 M -1)
1960000
Excitation (nm)565
Emission (nm)721
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageRefrigerated (2-8 °C); Minimize light exposure
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Page updated on May 24, 2026