Products
Technologies
Applications
Services
Resources
Selection Guides
About
PE/XFD700 Anti-human CD45 Antibody
UCHL1
UCHL1 is an anti-human monoclonal antibody that forms an immune complex with the CD45ro antigen. CD45ro (sometimes called CD45RO) is a 180 kD transmembrane glycoprotein that is found on the surface of cells like granulocytes, macrophages and NK cells. In certain organisms, CD45 plays a role in the upregulation of protein kinase activity, is a positive regulator of tumor necrosis factor production and is a negative regulator of protein kinase activity. Additionally, it has been associated with key biological processes like dephosphorylation, particularly protein dephosphorylation. CD45 acts in key cellular pathways, for example, the regulation of receptor signaling pathway via JAK-STAT, B cell receptor signaling pathway and positive regulation of extrinsic apoptotic signaling pathway. From a research standpoint, it is of biological interest due to its association with important macromolecules/ligands like Src kinases, p56lck and p59fyn. CD45 is a very popular antibody target, with over 54000 publications in the last decade. CD45ro is often used in flow cytometry applications as a phenotypic marker for differentiation of cell types, specifically in the study of inhibitory molecules and immunology. This antibody was purified through affinity chromatography and conjugated to PE/XFD700 (ex/em = 566/721 nm). XFD700 is manufactured by AAT Bioquest, and it has a chemical structure similar to that of Alexa Fluor® 700 (Alexa Fluor® is the trademark of Thermo Fisher). It is compatible with the 561 nm laser and 695/40 nm bandpass filter (for example, as in the Agilent Technologies NovoCyte Advanteon).
| Catalog | Size | Price | Quantity |
|---|---|---|---|
| 104561P0 | 25 tests | Price | |
| 104561P1 | 100 tests | Price | |
| 104561P2 | 500 tests | Price |
Physical properties
| Molecular weight | N/A |
| Solvent | water |
Antibody properties
| Other names | CD45RO |
| Clone | UCHL1 |
| Host | Mouse |
| Isotype | Mouse IgG2a, κ |
| Reactivity | Human |
Spectral properties
| Extinction coefficient (cm -1 M -1) | 1960000 |
| Excitation (nm) | 565 |
| Emission (nm) | 721 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Refrigerated (2-8 °C); Minimize light exposure |
Documents
Contact us
| Telephone | |
| Fax | |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Technical Support | Contact us |
| Request quotation | Request |
| Purchase order | Send to sales@aatbio.com |
| Shipping | Standard overnight for United States, inquire for international |
Page updated on December 21, 2025