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AAT Bioquest

PE/XFD700 Anti-human CD57 Antibody *HI57a, XFD700 Same Structure to Alexa Fluor™ 700*

The HI57a monoclonal antibody binds with human CD57, a 100 - 115 kD transmembrane protein often expressed on the surface of T cells, natural killer cells, granulocytes and platelets. CD57 is associated with a variety of biologically interesting macromolecules/ligands, for example, P-Selectin and Laminin. CD57 is a fairly uncommon antibody target, with a little more than 3000 publications in the last decade. Even still, CD57 has a variety of applications in costimulatory molecules and immunology research, frequently serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to PE/XFD700 (ex/em = 566/721 nm). XFD700 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 700 (Alexa Fluor® is the trademark of ThermoFisher). It is compatible with the 561 nm laser and 695/40 nm bandpass filter (for example, as in the Agilent Technologies NovoCyte).

Spectrum

Conjugation

We provide custom conjugation services for this antibody (eg. labeling of cd57 antibody with HRP). A list of available labels can be found in the table below:

AFAF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
ProteinsHRP, Alkaline Phosphatase, Streptavidin
TandemsAPC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small MoleculesBiotin
Traditional DyesFITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluorUV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page here.
Page updated on September 17, 2024

Ordering information

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Additional ordering information

Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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Antibody properties

Other namesHNK-1; Leu-7
CloneHI57a
Hostmouse
Isotypemouse IgM
Reactivityhuman
Application

Spectral properties

Extinction coefficient (cm -1 M -1)

1960000

Excitation (nm)

565

Emission (nm)

721

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
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