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AAT Bioquest

PE/XFD700 Anti-human CD71 Antibody *HI160, XFD700 Same Structure to Alexa Fluor™ 700*

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Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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Spectral properties
Extinction coefficient (cm -1 M -1)1960000
Excitation (nm)565
Emission (nm)721
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

OverviewpdfSDSpdfProtocol


Other names
T9; Transferrin receptor; TFRC
Clone
HI160
Host
mouse
Isotype
mouse IgG2b
Reactivity
human
Application
Extinction coefficient (cm -1 M -1)
1960000
Excitation (nm)
565
Emission (nm)
721
HI160 is an anti-human monoclonal antibody that is specific for the CD71 antigen. CD71 (also known as Transferrin receptor protein 1) is a 95 kD member of the Transferrin receptor family that is located on the surface of cells such as endothelial cells and stem cells. In certain organisms, CD71 is a positive regulator of isotype switching, is involved in the positive regulation of T cell proliferation and acts to positively regulate B cell proliferation. From a research standpoint, it is of biological interest due to its association with important macromolecules/ligands such as Transferrin. CD71 is a fairly uncommon antibody target, with a little more than 4000 publications in the last decade. Even still, CD71 is commonly used in flow cytometry applications as a phenotypic marker for differentiation of cell types, especially in the study of immunology. This antibody was purified through affinity chromatography and conjugated to PE/XFD700 (ex/em = 566/721 nm). XFD700 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 700 (Alexa Fluor® is the trademark of ThermoFisher). It is compatible with the 561 nm laser and 695/40 nm bandpass filter (for example, as in the Agilent Technologies NovoCyte).

Spectrum


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spectrum

Spectral properties

Extinction coefficient (cm -1 M -1)1960000
Excitation (nm)565
Emission (nm)721

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Conjugation


We provide custom conjugation services for this antibody (eg. labeling of cd71 antibody with HRP). A list of available labels can be found in the table below:

AFAF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
ProteinsHRP, Alkaline Phosphatase, Streptavidin
TandemsAPC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small MoleculesBiotin
Traditional DyesFITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluorUV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page here.