Name: PerCP Anti-human CD3 Antibody *HIT3a*
Brand: AAT Bioquest
SKU: 100301T0
Availability: InStock

PerCP Anti-human CD3 Antibody

HIT3a

HIT3a is an anti-human monoclonal antibody that recognizes the CD3e antigen. CD3e (sometimes referred to as T cell antigen receptor complex or T3E) is a 20 kD member of the Ig superfamily that is found on the surface of cells like T cells. CD3 acts in essential cellular pathways, namely, the G protein-coupled receptor signaling pathway, apoptotic signaling pathway and T cell receptor signaling pathway. In addition, in certain organisms, it positively regulates T cell anergy, acts to positively regulate cell-matrix adhesion and is involved in the positive regulation of calcium-mediated signaling. From a research standpoint, it is of biological interest due to its association with essential macromolecules/ligands such as TCR. CD3 is a very popular antibody target, with over 80000 publications in the last decade. CD3e is typically used in flow cytometry applications as a phenotypic marker for differentiation of cell types, especially in the study of immunology. This antibody was purified through affinity chromatography and conjugated to PerCP (ex/em = 477/678 nm). It is compatible with the 488 nm laser and 693/37 nm bandpass filter (for example, as in the Miltenyi Biotec MACSQuant Analyzer 10).

Datasheet

SDS

COA

Catalog	Size	Price
100301T0	25 tests	Price
100301T1	100 tests	Price
100301T2	500 tests	Price

Conjugation

We provide custom conjugation services for this antibody (e.g. labeling of CD3e antibody with HRP). A list of available labels can be found in the table below:

Label Type	Available Labels
AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page

here

Physical properties

Molecular weight	N/A
Solvent	water

Antibody properties

Other names	T3E
Clone	HIT3a
Host	Mouse
Isotype	IgG2a
Reactivity	Human

Spectral properties

Correction factor (280 nm)	0.22
Extinction coefficient (cm ^-1 M ^-1)	406000
Excitation (nm)	477
Emission (nm)	678

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Refrigerated (2-8 °C); Minimize light exposure

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on July 28, 2026