PerCP Anti-human CD3 Antibody *HIT3b*
The HIT3b monoclonal antibody binds to human CD3e, a 20 kD single-pass type i membrane protein commonly located on the surface of nkt cells, tregs, thymocytes (differentiation dependent)s, thymocytes and t cells. In many organisms, CD3 positively regulates T cell anergy, is a promoter of peptidyl-tyrosine phosphorylation and enhances interferon-gamma production. Also, it is a component of vital cellular pathways, namely, the G protein-coupled receptor signaling pathway, cell surface receptor signaling pathway and negative regulation of smoothened signaling pathway. From a research standpoint, it is of biological interest due to its association with vital macromolecules/ligands such as TCR. CD3 is a very popular antibody target, with over 80000 publications in the last decade. CD3e is frequently used in flow cytometry applications as a phenotypic marker for differentiation of cell types, particularly in the study of immunology. This antibody was purified through affinity chromatography and conjugated to PerCP (ex/em = 477/678 nm). It is compatible with the 488 nm laser and 667/30 nm bandpass filter (for example, as in the Agilent Technologies NovoCyte Advanteon).
Spectrum
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Conjugation
We provide custom conjugation services for this antibody (eg. labeling of cd3e antibody with HRP). A list of available labels can be found in the table below:
For additional information about custom conjugations, please consult our services page here.
AF | AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750 |
Proteins | HRP, Alkaline Phosphatase, Streptavidin |
Tandems | APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5 |
Small Molecules | Biotin |
Traditional Dyes | FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5 |
iFluor | 350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7 |
mFluor | UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780 |
For additional information about custom conjugations, please consult our services page here.
Page updated on December 13, 2024