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PerCP Anti-human CD3 Antibody *HIT3b*

The HIT3b monoclonal antibody binds to human CD3e, a 20 kD single-pass type i membrane protein commonly located on the surface of nkt cells, tregs, thymocytes (differentiation dependent)s, thymocytes and t cells. In many organisms, CD3 positively regulates T cell anergy, is a promoter of peptidyl-tyrosine phosphorylation and enhances interferon-gamma production. Also, it is a component of vital cellular pathways, namely, the G protein-coupled receptor signaling pathway, cell surface receptor signaling pathway and negative regulation of smoothened signaling pathway. From a research standpoint, it is of biological interest due to its association with vital macromolecules/ligands such as TCR. CD3 is a very popular antibody target, with over 80000 publications in the last decade. CD3e is frequently used in flow cytometry applications as a phenotypic marker for differentiation of cell types, particularly in the study of immunology. This antibody was purified through affinity chromatography and conjugated to PerCP (ex/em = 477/678 nm). It is compatible with the 488 nm laser and 667/30 nm bandpass filter (for example, as in the Agilent Technologies NovoCyte Advanteon).

Spectrum

Conjugation

We provide custom conjugation services for this antibody (eg. labeling of cd3e antibody with HRP). A list of available labels can be found in the table below:

AFAF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
ProteinsHRP, Alkaline Phosphatase, Streptavidin
TandemsAPC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small MoleculesBiotin
Traditional DyesFITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluorUV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page here.
Page updated on December 13, 2024

Ordering information

Price
Unit size
25 tests
100 tests
500 tests
Catalog Number
100311T0100311T1100311T2
Quantity
Add to cart

Additional ordering information

Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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Purchase orderSend to sales@aatbio.com
ShippingStandard overnight for United States, inquire for international
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Antibody properties

Other namesT3E
CloneHIT3b
Hostmouse
Isotypemouse IgG1
Reactivityhuman
Application

Spectral properties

Correction Factor (280 nm)

0.22

Extinction coefficient (cm -1 M -1)

406000

Excitation (nm)

477

Emission (nm)

678

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
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