Name: PerCP Anti-human CD3 Antibody *UCHT1*
Brand: AAT Bioquest
SKU: 100321T0
Availability: InStock

PerCP Anti-human CD3 Antibody

UCHT1

The UCHT1 monoclonal antibody binds to human CD3e, a 20 kD member of the Ig superfamily commonly found on the surface of tregs, thymocytes, nkt cells and thymocytes (differentiation dependent)s. In many organisms, CD3 enhances interleukin-4 production, is an inhibitor of gene expression and negatively regulates smoothened signaling pathway. Also, it plays a role in essential cellular pathways, for instance, the cell surface receptor signaling pathway, T cell receptor signaling pathway and apoptotic signaling pathway. From a research standpoint, it is of biological interest due to its association with vital macromolecules/ligands like TCR. CD3 is a very popular antibody target, with over 80000 publications in the last decade. CD3e is typically used in flow cytometry applications as a phenotypic marker for differentiation of cell types, particularly in the study of immunology. This antibody was purified through affinity chromatography and conjugated to PerCP (ex/em = 477/678 nm). It is compatible with the 488 nm laser and 702/87 nm bandpass filter (for example, as in the Luminex Amnis CellStream).

Protocol

SDS

COA

Catalog	Size	Price
100321T0	25 tests	Price
100321T1	100 tests	Price
100321T2	500 tests	Price

Conjugation

We provide custom conjugation services for this antibody (e.g. labeling of cd3e antibody with HRP). A list of available labels can be found in the table below:

Label Type	Available Labels
AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page

here

Physical properties

Molecular weight	N/A
Solvent	water

Antibody properties

Other names	T3E
Clone	UCHT1
Host	mouse
Reactivity	human

Spectral properties

Correction factor (280 nm)	0.22
Extinction coefficient (cm ^-1 M ^-1)	406000
Excitation (nm)	477
Emission (nm)	678

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Refrigerated (2-8 °C); Minimize light exposure

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 27, 2025