PerCP Anti-human/monkey CD20 Antibody 2H7

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Spectral properties

Correction Factor (280 nm)	0.22
Extinction coefficient (cm ^-1 M ^-1)	406000
Excitation (nm)	477
Emission (nm)	678

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22

Overview SDS Protocol

Other names

Bp35; B1

Clone

2H7

Host

mouse

Isotype

mouse igg2b, κ

Reactivity

human; monkey

Application

Correction Factor (280 nm)

0.22

Extinction coefficient (cm ^-1 M ^-1)

406000

Excitation (nm)

477

Emission (nm)

678

The 2H7 monoclonal antibody binds with human/monkey CD20, a 33 - 37 kD transmembrane glycoprotein commonly expressed on the surface of T cells and B cells. CD20 is a component of essential cellular pathways, in particular, the B cell receptor signaling pathway. From a research standpoint, it is of biological interest due to its association with vital macromolecules/ligands such as Lyn. CD20 is a moderately popular antibody target, with over 20000 publications in the last decade. CD20 is vital to immunology research, frequently serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to PerCP (ex/em = 477/678 nm). It is compatible with the 488 nm laser and 695/40 nm bandpass filter (for example, as in the BD FACSAria™ Fusion).

Spectrum

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Spectral properties

Correction Factor (280 nm)	0.22
Extinction coefficient (cm ^-1 M ^-1)	406000
Excitation (nm)	477
Emission (nm)	678

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Conjugation

We provide custom conjugation services for this antibody (eg. labeling of cd20 antibody with HRP). A list of available labels can be found in the table below:

AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780