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AAT Bioquest

PerCP/Cy5.5 Anti-human CD11a Antibody *HI111*

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Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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Spectral properties
Extinction coefficient (cm -1 M -1)350000
Excitation (nm)482
Emission (nm)695
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

OverviewpdfSDSpdfProtocol


Other names
LFA-1A; Integrin aL; ITGAL
Clone
HI111
Host
mouse
Isotype
mouse IgG1
Reactivity
human
Application
Extinction coefficient (cm -1 M -1)
350000
Excitation (nm)
482
Emission (nm)
695
HI111 is an anti-human monoclonal antibody that forms an immune complex with the CD11a antigen. CD11a (also known as Integrin alpha-L or LFA-1α chain) is a 170 - 180 kD transmembrane protein that is expressed on the surface of cells such as granulocytes and macrophages. CD11a has been associated with critical biological processes like cell-cell adhesion, especially leukocyte cell-cell adhesion. Moreover, it is a member of essential cellular pathways, for instance, the integrin-mediated signaling pathway. From a research standpoint, it is of biological interest due to its association with vital macromolecules/ligands such as CD18 and ICAM-1, 2, 3 and 4. CD11a is a fairly uncommon antibody target, with a little more than 3700 publications in the last decade. Even still, CD11a has a variety of applications in neuroscience and innate immunity research, typically serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to PerCP/Cy5.5 (ex/em = 489/679 nm). It is compatible with the 488 nm laser and 700/54 nm bandpass filter (for example, as in the BD FACSMelody™).

Spectrum


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spectrum

Spectral properties

Extinction coefficient (cm -1 M -1)350000
Excitation (nm)482
Emission (nm)695

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Conjugation


We provide custom conjugation services for this antibody (eg. labeling of cd11a antibody with HRP). A list of available labels can be found in the table below:

AFAF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
ProteinsHRP, Alkaline Phosphatase, Streptavidin
TandemsAPC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small MoleculesBiotin
Traditional DyesFITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluorUV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page here.