Name: PerCP/Cy5.5 Anti-human CD142 Antibody *HTF-1*
Brand: AAT Bioquest
SKU: 114201T0
Availability: InStock

PerCP/Cy5.5 Anti-human CD142 Antibody

HTF-1

HTF-1 is an anti-human monoclonal antibody that is specific for the CD142 antigen. CD142 (sometimes referred to as Tissue factor or F3) is a 45 kD single-pass type I membrane protein that is found on the surface of cells such as endothelial cells, epithelial cells and macrophages. CD142 has been closely linked to key biological processes like blood coagulation, specifically activation of blood coagulation via clotting cascade. Furthermore, it plays a role in vital cellular pathways, in particular, the cytokine-mediated signaling pathway, positive regulation of platelet-derived growth factor receptor signaling pathway and blood coagulation, extrinsic pathway. In certain organisms, CD142 promotes angiogenesis, upregulates endothelial cell proliferation and is involved in the positive regulation of cell migration. From a research standpoint, it is of biological interest due to its association with important macromolecules/ligands like factor Xa/TFPI and factor VIIa. CD142 is a relatively rare antibody target, with fewer than 200 publications in the last decade. Even still, CD142 is vital to angiogenesis research, typically serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to PerCP/Cy5.5 (ex/em = 489/679 nm). It is compatible with the 488 nm laser and 667/30 nm bandpass filter (for example, as in the Agilent Technologies NovoCyte Advanteon).

Datasheet

SDS

COA

Catalog	Size	Price
114201T0	25 tests	Price
114201T1	100 tests	Price
114201T2	500 tests	Price

Conjugation

We provide custom conjugation services for this antibody (e.g. labeling of CD142 antibody with HRP). A list of available labels can be found in the table below:

Label Type	Available Labels
AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page

here

Antibody properties

Other names	Tissue factor, F3
Clone	HTF-1
Host	Mouse
Isotype	IgG1, kappa
Reactivity	Human

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	350000
Excitation (nm)	482
Emission (nm)	695

Usage and storage

Intended use

Research Use Only (RUO)

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Page updated on August 19, 2026