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PerCP/Cy5.5 Anti-human CD3 Antibody
OKT-3
OKT-3 is an anti-human monoclonal antibody that targets the CD3e antigen. CD3e (alternatively called T cell antigen receptor complex or TCRE) is a 20 kD member of the Ig superfamily that is located on the surface of cells like T cells. CD3 is a member of important cellular pathways, in particular, the cell surface receptor signaling pathway, T cell receptor signaling pathway and negative regulation of smoothened signaling pathway. Furthermore, in certain organisms, it positively regulates calcium-mediated signaling, upregulates peptidyl-tyrosine phosphorylation and enhances cell-matrix adhesion. From a research standpoint, it is of biological interest due to its association with vital macromolecules/ligands such as TCR. CD3 is a very popular antibody target, with over 80000 publications in the last decade. CD3e is vital to immunology research, typically serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to PerCP/Cy5.5 (ex/em = 489/679 nm). It is compatible with the 488 nm laser and 695/40 nm bandpass filter (for example, as in the Thermo Fisher Attune NxT).
(Top) Spectral emission profiles generated using four spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm). Each laser produced a distinct emission pattern, and their combination yielded the composite spectral signature. (Bottom) Flow cytometry analysis of whole blood stained with PerCP/Cy5.5 Anti-human CD3 Antibody *OKT-3*. The fluorescence signal was monitored using an Cytek Aurora flow cytometer in the specific B9-A channel.
(Top) Spectral emission profiles generated using four spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm). Each laser produced a distinct emission pattern, and their combination yielded the composite spectral signature. (Bottom) Flow cytometry analysis of whole blood stained with PerCP/Cy5.5 Anti-human CD3 Antibody *OKT-3*. The fluorescence signal was monitored using an Cytek Aurora flow cytometer in the specific B9-A channel.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
100341U025 tests
Price
100341U1100 tests
Price
100341U2500 tests
Price
 
Antibody properties
Other namesT3E
CloneOKT-3
Hostmouse
Isotypemouse igg2a, κ
Reactivityhuman
Spectral properties
Extinction coefficient (cm -1 M -1)
350000
Excitation (nm)482
Emission (nm)695
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
Documents
Protocol
Safety Data Sheet
Certificate of Analysis
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Page updated on December 7, 2025