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AAT Bioquest

PerCP/Cy5.5 Anti-human CD45 Antibody *HI100*

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Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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Spectral properties
Extinction coefficient (cm -1 M -1)350000
Excitation (nm)482
Emission (nm)695
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

OverviewpdfSDSpdfProtocol


Other names
PTPRC
Clone
HI100
Host
mouse
Isotype
Mouse IgG2b, κ
Reactivity
human
Application
Extinction coefficient (cm -1 M -1)
350000
Excitation (nm)
482
Emission (nm)
695
HI100 is an anti-human monoclonal antibody that recognizes the CD45ra antigen. CD45ra (sometimes referred to as LY5, PTPRC, GP180 or LCA) is a 205 - 220 kD glycoprotein that is located on the surface of cells such as T cells and B cells. CD45 has been thought to be involved with essential biological processes like dephosphorylation, particularly protein dephosphorylation. Additionally, in certain organisms, it represses microglial cell activation, is involved in the positive regulation of humoral immune response mediated by circulating immunoglobulin and plays a role in the upregulation of extrinsic apoptotic signaling pathway. CD45 is a member of important cellular pathways, in particular, the cell surface receptor signaling pathway, positive regulation of antigen receptor-mediated signaling pathway and T cell receptor signaling pathway. From a research standpoint, it is of biological interest due to its association with vital macromolecules/ligands such as Src kinases. CD45 is a very popular antibody target, with over 54000 publications in the last decade. CD45ra is often used in flow cytometry applications as a phenotypic marker for differentiation of cell types, especially in the study of neuroscience, inhibitory molecules and immunology. This antibody was purified through affinity chromatography and conjugated to PerCP/Cy5.5 (ex/em = 489/679 nm). It is compatible with the 488 nm laser and 695/40 nm bandpass filter (for example, as in the BD FACSAria™ II).

Spectrum


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spectrum

Spectral properties

Extinction coefficient (cm -1 M -1)350000
Excitation (nm)482
Emission (nm)695

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Conjugation


We provide custom conjugation services for this antibody (eg. labeling of cd45ra antibody with HRP). A list of available labels can be found in the table below:

AFAF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
ProteinsHRP, Alkaline Phosphatase, Streptavidin
TandemsAPC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small MoleculesBiotin
Traditional DyesFITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluorUV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page here.