Portelite™ Fluorimetric Lithium Ion Quantification Kit

Portelite™ Fluorimetric Lithium Ion Quantification Kit is a fluorimetric assay kit for rapidly quantifying lithium ions in small-volume samples.

Detection Principle: Lithiumighty™ 520 increases fluorescence upon lithium binding.
Selectivity: Provides high selectivity for lithium ions.
Sample Advantage: Requires only a small sample volume.
Workflow: 5–10 minute room-temperature incubation.
Instrument Compatibility: Optimized for CytoCite™ and Qubit™ fluorometers at Ex/Em 480/530 nm.

The lithium standard curve was generated using the Portelite™ Fluorimetric Lithium Ion Quantification Kit.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
21353	50 Tests	Price

Overview

Portelite™ Fluorimetric Lithium Ion Quantification Kit™ uses our robust lithium ion indicator dye, Lithiumighty™ 520, which exhibit great fluorescence intensity enhancement upon binding to Lithium ions. Lithiumighty™ 520 is the most robust lithium ion indicator with high selectivity. It enables the kit to be useful for the rapid determination of lithium concentrations in a variety of samples compared to the other commercial lithium ion assays. This nanodrop-based assay kit requires a small amount of sample, it is particularly suitable for the determination of lithium ion concentration in fields or on site. Quantifying lithium ions is important in various scientific fields and industries, including biochemistry, medicine, environmental analysis, and food science etc. The rapid and accurate determination of lithium ions is particularly important in the battery industry. There are several methods commonly used to quantify lithium ions, including flame photometry, ion-selective electrodes (ISE), atomic absorption spectroscopy and fluorescence spectrophotometry. Flame photometry and atomic absorption spectroscopy require the inflammation of the samples. They are tedious to use and require expensive and sophisticated instrumentation. Ion-selective electrodes require large volumes of samples and often have low selectivity. Among all the methods, fluorescence spectrophotometry is the most convenient method for quantifying lithium ions. Fluorescence spectrophotometry involves complexing lithium ions with specific reagents and measuring the resulting fluorescence changes. However, there is still a lack of a fluorescence-based lithium ion assay kit in the commercial market due to the absence of a robust fluorescence lithium ion indicator. For the first time, Lithiumighty™ 520 filled this gap. It is the best fluorescent lithium ion indicator for rapidly determining lithium ion concentration in combination with a fluorescence device such as a fluorescence Nanodrop spectrophotometer or a fluorescence microplate reader.

References

View all 25 references: Citation Explorer

Advances in Therapeutic Monitoring of Lithium in the Management of Bipolar Disorder.

Authors:

Sheikh, Mahsa and Qassem, Meha and Triantis, Iasonas F and Kyriacou, Panicos A

Journal:

Sensors (Basel, Switzerland) (2022)

Biological properties of benzopyran-based platinum (II) complexes.

Authors:

Malinowska, Katarzyna and Modranka, Roman and Majsterek, Ireneusz and Misiak, Piotr

Journal:

Polski przeglad chirurgiczny (2014): 172-6

Evaluation of lithium determination in three analyzers: flame emission, flame atomic absorption spectroscopy and ion selective electrode.

Authors:

Aliasgharpour, Mehri and Hagani, Hamid

Journal:

North American journal of medical sciences (2009): 244-6

Superiority of nitric acid for deproteinization in the determination of trace lithium in serum by graphite furnace atomic absorption spectrometry.

Authors:

Zhao, Jianxing and Gao, Pingjin and Wu, Shengnan and Zhu, Dingliang

Journal:

Journal of pharmaceutical and biomedical analysis (2009): 1075-9

Mineral salts removal and lithium traces determination in highly concentrated solutions and natural brines.

Authors:

Hamzaoui, Hichem and M'nif, Adel and Rokbani, Ridha

Journal:

Talanta (2006): 847-51

Spectral properties

Excitation (nm)	491
Emission (nm)	513

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Qubit Fluorometer
Excitation	480 nm
Emission	530 nm
Instrument specification(s)	0.2 mL PCR tube

CytoCite Fluorometer
Excitation	480 nm
Emission	530 nm
Instrument specification(s)	0.2 mL PCR tube

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Page updated on October 25, 2026

Component A: Lithiumighty™ 520	1 Vial
Component B: Assay Buffer	1 Bottle (20 mL)
Component C: Lithium Standard	1 Vial (15 mg)
Component D: DMSO	1 Vial (200 µL)