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Propidium iodide *CAS 25535-16-4*

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Physical properties
Molecular weight668.39
Spectral properties
Extinction coefficient (cm -1 M -1)60001
Excitation (nm)537
Emission (nm)618
Quantum yield0.21
Storage, safety and handling
Certificate of OriginDownload PDF
H-phraseH303, H313, H340
Hazard symbolT
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R68
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
Extinction coefficient (cm -1 M -1)
Excitation (nm)
Emission (nm)
Quantum yield
Propidium iodide (PI) belongs to the same chemical class of ethidium bromide. As in the case of ethidium bromide its fluorescence is enhanced for 20-30-fold upon binding to nucleic acids. The fluorescence excitation maximum is red-shifted for 30-40 nm and the fluorescence emission maximum blue-shifted for 15 nm or so. PI also binds to RNA as DAPI and acridine orange. PI is commonly used for identifying dead cells in a population of cells and as a counterstain in multicolor fluorescent techniques. It can also be used to differentiate necrotic, apoptotic and normal cells. It is suitable for fluorescence microscopy, flow cytometry and fluorometry.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Propidium iodide *CAS 25535-16-4* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM149.613 µL748.066 µL1.496 mL7.481 mL14.961 mL
5 mM29.923 µL149.613 µL299.226 µL1.496 mL2.992 mL
10 mM14.961 µL74.807 µL149.613 µL748.066 µL1.496 mL

Molarity calculator

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Spectral properties

Extinction coefficient (cm -1 M -1)60001
Excitation (nm)537
Emission (nm)618
Quantum yield0.21

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View all 60 references: Citation Explorer
Analysis of cycloheximide-induced apoptosis in human leukocytes: Fluorescence microscopy using annexin V/propidium iodide versus acridin orange/ethidium bromide
Authors: Baskic D, Popovic S, Ristic P, Arsenijevic NN.
Journal: Cell Biol Int (2006): 924
Prognostic significance of plasma cell propidium iodide and annexin-V indices and their mutual ratio in multiple myeloma
Authors: Scudla V, Ordeltova M, Minarik J, Dusek L, Zemanova M, Bacovsky J.
Journal: Neoplasma (2006): 213
Assessment of the cell viability of cultured Perkinsus marinus (Perkinsea), a parasitic protozoan of the Eastern oyster, Crassostrea virginica, using SYBRgreen-propidium iodide double staining and flow cytometry
Authors: Soudant P, Chu FL, Lund ED.
Journal: J Eukaryot Microbiol (2005): 492
Evaluation of plasma cell propidium-iodide and annexin-V indices: Their relation to prognosis in multiple myeloma
Authors: Minarik J, Scudla V, Ordeltova M, Bacovsky J, Zemanova M.
Journal: Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub (2005): 271
Low propidium iodide intensity in flow cytometric white blood cell counting as a marker of cell destruction
Authors: Wagner T, Guber SE, Stubenrauch ML, Lanzer G, Neumueller J.
Journal: Transfusion (2005): 228
Propidium iodide and PicoGreen as dyes for the DNA fluorescence correlation spectroscopy measurements
Authors: Kral T, Widerak K, Langner M, Hof M.
Journal: J Fluoresc (2005): 179
The assessment of viability in isolated rat hepatocytes subjected to cold or subzero non-freezing preservation protocols using a propidium iodide modified test
Authors: Giraudi PJ, Almada LL, Mamprin ME, Guibert EE, Furno G, Rodriguez JV.
Journal: Cryo Letters (2005): 169
Single UV excitation of Hoechst 33342 and propidium iodide for viability assessment of rhesus monkey spermatozoa using flow cytometry
Authors: Cai K, Yang J, Guan M, Ji W, Li Y, Rens W.
Journal: Arch Androl (2005): 371
Green fluorescent protein-propidium iodide (GFP-PI) based assay for flow cytometric measurement of bacterial viability
Authors: Lehtinen J, Nuutila J, Lilius EM.
Journal: Cytometry A (2004): 165
Evaluation of cytotoxic function and apoptosis in interleukin (IL)-12/IL-15-treated umbilical cord or adult peripheral blood natural killer cells by a propidium-iodide based flow cytometry
Authors: Lin SJ, Cheng PJ, Huang YJ, Kuo ML.
Journal: Pediatr Allergy Immunol (2004): 79