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Propidium iodide *1 mg/mL aqueous solution*

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Physical properties
Molecular weight668.39
Spectral properties
Extinction coefficient (cm -1 M -1)60001
Excitation (nm)537
Emission (nm)618
Quantum yield0.21
Storage, safety and handling
Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


See also: Nucleus
Molecular weight
Extinction coefficient (cm -1 M -1)
Excitation (nm)
Emission (nm)
Quantum yield
Propidium iodide (PI) belongs to the same chemical class of ethidium bromide. As in the case of ethidium bromide its fluorescence is enhanced for 20-30-fold upon binding to nucleic acids. The fluorescence excitation maximum is red-shifted for 30–40 nm and the fluorescence emission maximum blue-shifted for 15 nm or so. PI also binds to RNA as DAPI and acridine orange. PI is commonly used for identifying dead cells in a population of cells and as a counterstain in multicolor fluorescent techniques. It can also be used to differentiate necrotic, apoptotic and normal cells. It is suitable for fluorescence microscopy, flow cytometry and fluorometry.


Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Propidium iodide *1 mg/mL aqueous solution* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM149.613 µL748.066 µL1.496 mL7.481 mL14.961 mL
5 mM29.923 µL149.613 µL299.226 µL1.496 mL2.992 mL
10 mM14.961 µL74.807 µL149.613 µL748.066 µL1.496 mL

Molarity calculator

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Spectral properties

Extinction coefficient (cm -1 M -1)60001
Excitation (nm)537
Emission (nm)618
Quantum yield0.21

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View all 1 citations: Citation Explorer
Microfluidic System for Cell Mixing and Particle Focusing Using Dean Flow Fractionation
Authors: Wiede, Alexander and Stranik, Ondrej and Tannert, Astrid and Neugebauer, Ute
Journal: (2023): 671--685


View all 50 references: Citation Explorer
Detection of gamma radiation processed onion during storage using propidium iodide based fluorescence microscopy.
Authors: Sharma, Tanmayee and Kavita, and Mishra, Bibhuti Bhusan and Variyar, Prasad Shekhar
Journal: Food chemistry (2023): 133928
Replacement of DAPI with propidium iodide could extend the utilisation of archival tissue samples for immunofluorescent techniques.
Authors: Cizkova, Katerina and Koubova, Katerina and Tauber, Zdenek
Journal: Histopathology (2022): 409-411
Corrigendum to: The DNA intercalators ethidium bromide and propidium iodide also bind to core histones.
Journal: FEBS open bio (2022): 1087
Rapid Determination of Antibiotic Resistance in Klebsiella pneumoniae by a Novel Antibiotic Susceptibility Testing Method Using SYBR Green I and Propidium Iodide Double Staining.
Authors: Zhang, Yabin and Fan, Weihua and Shao, Chunhong and Wang, Jiajia and Jin, Yan and Shao, Jing and Zhang, Ying and Wang, Yong
Journal: Frontiers in microbiology (2021): 650458
Propidium iodide enabled live imaging of Pasteuria sp.-Pratylenchus zeae infection studies under fluorescence microscopy.
Authors: Perrine-Walker, Francine and Le, Khoa
Journal: Protoplasma (2021): 279-287
Ultra-Rapid Drug Susceptibility Testing for Klebsiella pneumoniae Clinical Isolates in 60 Min by SYBR Green I/Propidium Iodide Viability Assay.
Authors: Chen, Jiazhen and Wang, Xuyang and Wang, Shiyong and Chen, Chen and Zhang, Wenhong and Zhang, Ying
Journal: Frontiers in microbiology (2021): 694522
An Annexin V-FITC-Propidium Iodide-Based Method for Detecting Apoptosis in a Non-Small Cell Lung Cancer Cell Line.
Authors: Kumar, Robin and Saneja, Ankit and Panda, Amulya K
Journal: Methods in molecular biology (Clifton, N.J.) (2021): 213-223
Evaluation of the use of SYBR-14 and propidium iodide stain in a fluorescent computer-assisted spermatozoal quantification method in dogs.
Authors: Watts, John R
Journal: Reproduction in domestic animals = Zuchthygiene (2021): 89-102
Optimization of staining with SYTO 9/propidium iodide: interplay, kinetics and impact on Brevibacillus brevis.
Authors: Deng, Ying and Wang, Lili and Chen, Yujia and Long, Yan
Journal: BioTechniques (2020): 88-98
Rapid determination of carbapenem resistance by low-cost colorimetric methods: Propidium Iodide and alamar blue staining.
Authors: Choi, Jiyoon and Baek, Jiwon and Kweon, Daehyuk and Ko, Kwan Soo and Yoon, Hyunjin
Journal: Journal of microbiology (Seoul, Korea) (2020): 415-421