Protonex™Green 500-Latex Bead Conjugate

Protonex™ Green 500-Latex Bead Conjugates exhibit distinctive pH-dependent fluorescence characteristics, becoming significantly more fluorescent in acidic conditions, unlike most conventional fluorescent dyes that increase in fluorescence at higher pH levels. This unique feature makes them highly suitable for investigating phagocytosis and its modulation by various drugs or environmental factors. The beads show minimal fluorescence outside of cells, eliminating the need for wash steps and enhancing their suitability for live-cell imaging. Protonex™ Green 500-Latex Bead Conjugates are particularly effective in highlighting acidic cellular compartments such as phagosomes, lysosomes, and endosomes, where they emit bright green fluorescence. Additionally, these conjugates can be used in combination with red fluorescent dyes like RFP, Calbryte™ 630 calcium dye, calcein red, or Cy5-labeled antibodies for multiplexed cell functional analysis. Their spectral properties are similar to those of FITC, allowing for the use of common FITC filter sets in assays involving Protonex™ Green 500-Latex Bead Conjugates, further facilitating their integration into existing experimental setups.