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Protonex™Green 500-Latex Bead Conjugate
Product key features
The Protonex™ Green 500-Latex Bead Conjugate provides a ready-to-use, pH-sensitive fluorescent tool for monitoring phagocytosis and intracellular acidification processes in live cells.
• pH-activated fluorescence: Non-fluorescent at neutral pH and becomes strongly fluorescent in acidic compartments such as phagosomes and phagolysosomes.
• Latex-based targeting: Composed of uniform, synthetic latex beads that offer consistent size and surface properties for controlled phagocytic uptake.
• Multipurpose reagent: Suitable for incorporation into custom assay workflows for microscopy, flow cytometry, or plate-based readouts.
Product description
Example protocol
AT A GLANCE
Solvent: | Water |
Solids Content: | 1% in PBS |
Number of Microspheres per mL: | ~4e+10 |
Ex/Em: | 445/503 nm |
Mean Diameter: | 0.72 µm |
SAMPLE EXPERIMENTAL PROTOCOL
The following is a recommended protocol for granulocytes. This protocol only provides a guideline and should be modified
according to your specific experimental conditions.
Prepare cells as desired. For example, prepare the granulocytes at 107 cells/mL with Hanks and 20 mM Hepes buffer (HHBS), and add 100 μL to a polypropylene tube.
Note: Each cell line should be evaluated on an individual basis to determine the optimal cell density.
Add 1-10 μL of the Protonex™ Green 500-Latex Bead Conjugate to the tube and incubate with gentle shaking for 30 minutes at 37˚C.
Note: Each cell line should be evaluated on an individual basis to determine the optimal incubation time.
Prepare an identical sample that is incubated at 4˚C and label it as a control.
At the end of the 30-minute incubation, stop phagocytosis by adding 2mL of ice-cold HHBS and mix well.
Wash the cells 2 times with cold HBSS.
Resuspend the cells in 500 μL of cold HBSS, keep the samples at 4˚C, and analyze immediately using a fluorescence microscope equipped with a FITC filter set.
Note: For fluorescence microplate readers, monitor the fluorescence intensity at Ex/Em = 450/510 nm (Cutoff = 490 nm).
Spectrum
References
Authors: Liu, Pei and Jiang, Pengfei and Yu, Yunfeng and Tan, Kang and Qin, Gen-Yan and Liu, Tingting and Tian, Sainan and Peng, Jun and Peng, Qinghua
Journal: Journal of ethnopharmacology (2024): 117544
Authors: Zhang, Sasa and Liu, Xing and Li, Cui and Wang, Qian and Yang, Shanshan and Peng, Xudong and Hu, Liting and Zhao, Guiqiu and Lin, Jing
Journal: Current eye research (2024): 25-32
Authors: Moreno, Jonathan and Zoghebi, Khalid and Salehi, David and Kim, Lois and Shoushtari, Sorour Khayyatnejad and Tiwari, Rakesh K and Parang, Keykavous
Journal: Pharmaceuticals (Basel, Switzerland) (2023)
Authors: Li, You-Xian and Chen, Alvin and Leu, Wei-Ming
Journal: International journal of molecular sciences (2023)
Authors: Jezierski, Anna and Huang, Jez and Haqqani, Arsalan S and Haukenfrers, Julie and Liu, Ziying and Baumann, Ewa and Sodja, Caroline and Charlebois, Claudie and Delaney, Christie E and Star, Alexandra T and Liu, Qing and Stanimirovic, Danica B
Journal: Fluids and barriers of the CNS (2023): 36
Safety data sheet