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Protonex™Green 500-Latex Bead Conjugate
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Spectral properties
| Extinction coefficient (cm -1 M -1) | 4000 |
| Excitation (nm) | 445 |
| Emission (nm) | 503 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Refrigerated (2-8 °C); Minimize light exposure |
| UNSPSC | 12352200 |
| Overview |  SDSProtocol |
Extinction coefficient (cm -1 M -1) 4000 | Excitation (nm) 445 | Emission (nm) 503 |
Protonex™ Green 500-Latex Bead Conjugates exhibit distinctive pH-dependent fluorescence characteristics, becoming significantly more fluorescent in acidic conditions, unlike most conventional fluorescent dyes that increase in fluorescence at higher pH levels. This unique feature makes them highly suitable for investigating phagocytosis and its modulation by various drugs or environmental factors. The beads show minimal fluorescence outside of cells, eliminating the need for wash steps and enhancing their suitability for live-cell imaging. Protonex™ Green 500-Latex Bead Conjugates are particularly effective in highlighting acidic cellular compartments such as phagosomes, lysosomes, and endosomes, where they emit bright green fluorescence. Additionally, these conjugates can be used in combination with red fluorescent dyes like RFP, Calbryte™ 630 calcium dye, calcein red, or Cy5-labeled antibodies for multiplexed cell functional analysis. Their spectral properties are similar to those of FITC, allowing for the use of common FITC filter sets in assays involving Protonex™ Green 500-Latex Bead Conjugates, further facilitating their integration into existing experimental setups.
Platform
Fluorescence microscope
| Excitation | FITC filter set |
| Emission | FITC filter set |
| Recommended plate | Black wall/clear bottom |
Example protocol
AT A GLANCE
Chemical and Physical Properties
Solvent: | Water |
Solids Content: | 1% in PBS |
Number of Microspheres per mL: | ~4e+10 |
Ex/Em: | 445/503 nm |
Mean Diameter: | 0.72 µm |
SAMPLE EXPERIMENTAL PROTOCOL
Important
The following is a recommended protocol for granulocytes. This protocol only provides a guideline and should be modified
according to your specific experimental conditions.
Protocol
Prepare cells as desired. For example, prepare the granulocytes at 107 cells/mL with Hanks and 20 mM Hepes buffer (HHBS), and add 100 μL to a polypropylene tube.
Note: Each cell line should be evaluated on an individual basis to determine the optimal cell density.
Add 1-10 μL of the Protonex™ Green 500-Latex Bead Conjugate to the tube and incubate with gentle shaking for 30 minutes at 37˚C.
Note: Each cell line should be evaluated on an individual basis to determine the optimal incubation time.
Prepare an identical sample that is incubated at 4˚C and label it as a control.
At the end of the 30-minute incubation, stop phagocytosis by adding 2mL of ice-cold HHBS and mix well.
Wash the cells 2 times with cold HBSS.
Resuspend the cells in 500 μL of cold HBSS, keep the samples at 4˚C, and analyze immediately using a fluorescence microscope equipped with a FITC filter set.
Note: For fluorescence microplate readers, monitor the fluorescence intensity at Ex/Em = 450/510 nm (Cutoff = 490 nm).
Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Extinction coefficient (cm -1 M -1) | 4000 |
| Excitation (nm) | 445 |
| Emission (nm) | 503 |
References
View all 50 references: Citation Explorer
Modified Danzhi Xiaoyao Powder (MDXP) improves the corneal damage in dry eye disease (DED) mice through phagocytosis.
Authors: Liu, Pei and Jiang, Pengfei and Yu, Yunfeng and Tan, Kang and Qin, Gen-Yan and Liu, Tingting and Tian, Sainan and Peng, Jun and Peng, Qinghua
Journal: Journal of ethnopharmacology (2024): 117544
Authors: Liu, Pei and Jiang, Pengfei and Yu, Yunfeng and Tan, Kang and Qin, Gen-Yan and Liu, Tingting and Tian, Sainan and Peng, Jun and Peng, Qinghua
Journal: Journal of ethnopharmacology (2024): 117544
Role of Inhibiting Inflammation of LC3-Associated Phagocytosis in Dry Eye Disease.
Authors: Zhang, Sasa and Liu, Xing and Li, Cui and Wang, Qian and Yang, Shanshan and Peng, Xudong and Hu, Liting and Zhao, Guiqiu and Lin, Jing
Journal: Current eye research (2024): 25-32
Authors: Zhang, Sasa and Liu, Xing and Li, Cui and Wang, Qian and Yang, Shanshan and Peng, Xudong and Hu, Liting and Zhao, Guiqiu and Lin, Jing
Journal: Current eye research (2024): 25-32
Pharmacokinetics, absorption and transport mechanism for ginseng polysaccharides.
Authors: Zhang, Juanhong and He, Jiaxin and Huang, Jumin and Li, Xuefeng and Fan, Xiaoqing and Li, Wenbin and Wu, Guofan and Xie, Chun and Fan, Xing-Xing and Zhang, Junmin and Yao, Xiaojun and Wang, Rong and Leung, Elaine Lai-Han
Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie (2023): 114610
Authors: Zhang, Juanhong and He, Jiaxin and Huang, Jumin and Li, Xuefeng and Fan, Xiaoqing and Li, Wenbin and Wu, Guofan and Xie, Chun and Fan, Xing-Xing and Zhang, Junmin and Yao, Xiaojun and Wang, Rong and Leung, Elaine Lai-Han
Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie (2023): 114610
Rapamycin treatment induces tubular proteinuria: role of megalin-mediated protein reabsorption.
Authors: Peres, Rodrigo A S and Peruchetti, Diogo B and Silva-Aguiar, Rodrigo P and Teixeira, Douglas E and Gomes, Carlos P and Takiya, Christina M and Pinheiro, Ana Acacia S and Caruso-Neves, Celso
Journal: Frontiers in pharmacology (2023): 1194816
Authors: Peres, Rodrigo A S and Peruchetti, Diogo B and Silva-Aguiar, Rodrigo P and Teixeira, Douglas E and Gomes, Carlos P and Takiya, Christina M and Pinheiro, Ana Acacia S and Caruso-Neves, Celso
Journal: Frontiers in pharmacology (2023): 1194816
TAT peptide at treatment-level concentrations crossed brain endothelial cell monolayer independent of receptor-mediated endocytosis or peptide-inflicted barrier disruption.
Authors: Wu, Meng-Chih and Wang, Eric Yuhsiang and Lai, Ted Weita
Journal: PloS one (2023): e0292681
Authors: Wu, Meng-Chih and Wang, Eric Yuhsiang and Lai, Ted Weita
Journal: PloS one (2023): e0292681
Synthesis and Anti-Angiogenic Activity of Novel c(RGDyK) Peptide-Based JH-VII-139-1 Conjugates.
Authors: Leonidis, George and Koukiali, Anastasia and Sigala, Ioanna and Tsimaratou, Katerina and Beis, Dimitris and Giannakouros, Thomas and Nikolakaki, Eleni and Sarli, Vasiliki
Journal: Pharmaceutics (2023)
Authors: Leonidis, George and Koukiali, Anastasia and Sigala, Ioanna and Tsimaratou, Katerina and Beis, Dimitris and Giannakouros, Thomas and Nikolakaki, Eleni and Sarli, Vasiliki
Journal: Pharmaceutics (2023)
PPARγ activation inhibits endocytosis of claudin-4 and protects against deoxynivalenol-induced intestinal barrier dysfunction in IPEC-J2 cells and weaned piglets.
Authors: Li, Enkai and Li, Chuang and Horn, Nathan and Ajuwon, Kolapo M
Journal: Toxicology letters (2023): 8-20
Authors: Li, Enkai and Li, Chuang and Horn, Nathan and Ajuwon, Kolapo M
Journal: Toxicology letters (2023): 8-20
Mouse embryonic stem cell-derived blood-brain barrier model: applicability to studying antibody triggered receptor mediated transcytosis.
Authors: Jezierski, Anna and Huang, Jez and Haqqani, Arsalan S and Haukenfrers, Julie and Liu, Ziying and Baumann, Ewa and Sodja, Caroline and Charlebois, Claudie and Delaney, Christie E and Star, Alexandra T and Liu, Qing and Stanimirovic, Danica B
Journal: Fluids and barriers of the CNS (2023): 36
Authors: Jezierski, Anna and Huang, Jez and Haqqani, Arsalan S and Haukenfrers, Julie and Liu, Ziying and Baumann, Ewa and Sodja, Caroline and Charlebois, Claudie and Delaney, Christie E and Star, Alexandra T and Liu, Qing and Stanimirovic, Danica B
Journal: Fluids and barriers of the CNS (2023): 36
Amphiphilic Cell-Penetrating Peptides Containing Arginine and Hydrophobic Residues as Protein Delivery Agents.
Authors: Moreno, Jonathan and Zoghebi, Khalid and Salehi, David and Kim, Lois and Shoushtari, Sorour Khayyatnejad and Tiwari, Rakesh K and Parang, Keykavous
Journal: Pharmaceuticals (Basel, Switzerland) (2023)
Authors: Moreno, Jonathan and Zoghebi, Khalid and Salehi, David and Kim, Lois and Shoushtari, Sorour Khayyatnejad and Tiwari, Rakesh K and Parang, Keykavous
Journal: Pharmaceuticals (Basel, Switzerland) (2023)
Macropinoscope: Real-Time Simultaneous Tracking of pH and Cathepsin B Activity in Individual Macropinosomes.
Authors: Hirose, Hisaaki and Nakata, Eiji and Zhang, Zhengxiao and Shibano, Yuya and Maekawa, Masashi and Morii, Takashi and Futaki, Shiroh
Journal: Analytical chemistry (2023): 11410-11419
Authors: Hirose, Hisaaki and Nakata, Eiji and Zhang, Zhengxiao and Shibano, Yuya and Maekawa, Masashi and Morii, Takashi and Futaki, Shiroh
Journal: Analytical chemistry (2023): 11410-11419