The amino-reactive Protonex™ Green 500 SE (#21216) was developed for preparing Protonex™ Green 500 conjugates as fluorescent pH probes. However, the utility of Protonex™ Green 500 SE was limited by its poor solubility. Protonex™ Green 500-PEG12 SE (#21219) has been developed to significantly improve the poor solubility of Protonex™ Green 500 SE (#21216) while maintaining the excellent spectral and pH properties of Protonex™ Green 500. They have identical spectra and pH profile while Green 500-PEG12 has significantly improved solubility. Protonex™ Green 500 dye demonstrated pH-dependent fluorescence. Unlike most of the existing fluorescent dyes that are more fluorescent at higher pH, acidic conditions enhance the fluorescence of Protonex™ Green 500 dye, making it an excellent acidotropic fluorescent probe. The fluorescence of Protonex™ Green 500 dye increases as pH decreases from neutral to the acidic. The lack of fluorescence outside the cell eliminates the wash steps. Protonex™ Green dye provides a powerful tool to monitor acidic cell compartments such as endosomes and lysosomes. Protonex™ Green dye is non-fluorescent outside the cells but fluoresces brightly green in acidic compartments (such as phagosomes, lysosomes and endosomes). This Protonex™ Green enables the specific detection of cellular acidic compartments with reduced signal variability and improved accuracy for imaging or flow applications. Protonex™ Green has the spectral properties similar to those of FITC, making the common filter set of FITC readily available to the assays of Protonex™ Green.
Catalog | Size | Price | Quantity |
---|---|---|---|
21219 | 1 mg | Price |
Molecular weight | 1139.26 |
Solvent | DMSO |
Excitation (nm) | 453 |
Emission (nm) | 522 |
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Telephone | |
Fax | |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Technical Support | Contact us |
Request quotation | Request |
Purchase order | Send to sales@aatbio.com |
Shipping | Standard overnight for United States, inquire for international |