Name: Protonex™ Green 500, SE
Brand: AAT Bioquest
SKU: 21216
Price: 232 USD
Availability: InStock

Protonex™ Green 500, SE

Protonex™ Green dye demonstrated pH-dependent fluorescence. Unlike most of the existing fluorescent dyes that are more fluorescent at higher pH, acidic conditions enhance the fluorescence of Protonex™ Green dye. The fluorescence of Protonex™ Green dye increases as pH decreases from neutral to the acidic. The lack of fluorescence outside the cell eliminates the wash steps. Protonex™ Green dye provides a powerful tool to monitor acidic cell compartments such as endosomes and lysosomes. Protonex™ Green dye is non-fluorescent outside the cells, but fluoresces brightly green in acidic compartments (such as phagosomes, lysosomes and endosomes). This Protonex™ Green enables the specific detection of cellular acidic compartments with reduced signal variability and improved accuracy for imaging or flow applications. Protonex™ Green has the spectral properties similar to those of FITC, making the common filter set of FITC readily available to the assays of Protonex™ Green. This amino-reactive Protonex™ Green derivative can be used for preparing Protonex™ Green conjugates as fluorescent pH probes.

The pH dependent Emission spectra of Protonex™ Green 500.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
21216	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Protonex™ Green 500, SE to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	185.343 µL	926.715 µL	1.853 mL	9.267 mL	18.534 mL
5 mM	37.069 µL	185.343 µL	370.686 µL	1.853 mL	3.707 mL
10 mM	18.534 µL	92.672 µL	185.343 µL	926.715 µL	1.853 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	539.54 g/mol	=	mL	x	mM	=	mmol

References

View all 56 references: Citation Explorer

Monitoring phospholipid dynamics during phagocytosis: application of genetically-encoded fluorescent probes

Authors:

Sarantis H, Grinstein S.

Journal:

Methods Cell Biol (2012): 429

Phagocytosis and digestion of pH-sensitive fluorescent dye (Eos-FP) transfected E. coli in whole blood assays from patients with severe sepsis and septic shock

Authors:

Schreiner L, Huber-Lang M, Weiss ME, Hohmann H, Schmolz M, Schneider EM.

Journal:

J Cell Commun Signal (2011): 135

The application of fluorescent probes for the analysis of lipid dynamics during phagocytosis

Authors:

Flannagan RS, Grinstein S.

Journal:

Methods Mol Biol (2010): 121

Quantification of microsized fluorescent particles phagocytosis to a better knowledge of toxicity mechanisms

Authors:

Leclerc L, Boudard D, Pourchez J, Forest V, Sabido O, Bin V, Palle S, Grosseau P, Bernache D, Cottier M.

Journal:

Inhal Toxicol (2010): 1091

Analysis of macrophage phagocytosis: quantitative assays of phagosome formation and maturation using high-throughput fluorescence microscopy

Authors:

Steinberg BE, Grinstein S.

Journal:

Methods Mol Biol (2009): 45

Physical properties

Molecular weight	539.54
Solvent	DMSO

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	4000
Excitation (nm)	445
Emission (nm)	503

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

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Page updated on October 12, 2025